湖南柑橘衰退病调查分析及弱毒系筛选  

Investigation of stem-pitting Citrus tristeza virus and screening of mild strains in Hunan Province

作　　者：李芳[1,2,3] 邓子牛[1,2,3] 韩健[4] 严佳文[1,2,3] 舒广平[2,4] 戴素明[1,2,3] 

机构地区：[1]湖南农业大学园艺园林学院,长沙410128 [2]国家柑橘改良中心长沙分中心,长沙410128 [3]湖南省作物种质创新与资源利用重点实验室,长沙410128 [4]湖南省园艺研究所,长沙410125

出　　处：《植物病理学报》2010年第4期442-445,共4页Acta Phytopathologica Sinica

基　　金：湖南农业大学青年基金项目(07QN36);湖南省科技计划重点项目(2008WK2007)

摘　　要：The investigation showed that stem-pitting Citrus tristeza virus(CTV)occurred commonly in citrus production areas in several varieties of Hunan Province.Accurate detection of CTV strains was performed by p23/PCR method,PCR and the results indicated that the most samples were infected with several CTV isolates.Three mild strains were isolated and their pathogenicity was identified by biological identification,it indicated that p23/PCR groups had uniformity with the pathogenicity of CTV isolates.Furthermore,three mild isolates were tested in the cross protection by analysis of biological symptoms and composition of p23 gene.Different protecting effects were observed among these strains and W17 mild isolate was effective.The investigation showed that stem-pitting Citrus tristeza virus（CTV）occurred commonly in citrus production areas in several varieties of Hunan Province.Accurate detection of CTV strains was performed by p23/PCR method,PCR and the results indicated that the most samples were infected with several CTV isolates.Three mild strains were isolated and their pathogenicity was identified by biological identification,it indicated that p23/PCR groups had uniformity with the pathogenicity of CTV isolates.Furthermore,three mild isolates were tested in the cross protection by analysis of biological symptoms and composition of p23 gene.Different protecting effects were observed among these strains and W17 mild isolate was effective.

关 键 词：柑橘衰退病毒 弱毒株 湖南省 筛选 virus 柑橘病害 柑橘产业 成功经验 

分 类 号：S436.661.19[农业科学—农业昆虫与害虫防治]