海洛因对大鼠海马神经元痛觉调制的影响  

Effects of heroin on regulation of painful perception in hippocampus neurons of rat

作　　者：徐国强[1] 潘际刚[1] 潘贵书[1] 李淑芳[2] 

机构地区：[1]贵阳医学院生理学教研室,贵阳550004 [2]贵阳医学院药理学教研室,贵阳550004

出　　处：《中华行为医学与脑科学杂志》2010年第8期687-688,共2页Chinese Journal of Behavioral Medicine and Brain Science

基　　金：贵州省教育厅自然科学基金（黔教科2005212）

摘　　要：目的 采用海洛因依赖大鼠模型来探讨海洛因成瘾对大鼠背侧和腹侧海马痛觉调制的影响.方法 按剂量逐日递增原则,1日2次,连续9d皮下注射海洛因建立其依赖SD大鼠模型.从第10天起,每天给予一次维持剂量的海洛因27 mg/kg,第14天用玻璃微电极细胞外分别记录依赖组和对照组大鼠背侧和腹侧海马神经元在鼠尾伤害性电刺激后的单位放电.结果 给予伤害性刺激后,海洛因依赖组大鼠背侧海马神经元以无影响效应（59.09%）为主,对照组海马神经元受痛刺激的调制的比例为66.67%,2组差异有显著性（P＜0.05）;而腹侧海马神经元对伤害性刺激呈激活、抑制、无影响反应的比例在对照组分别为20.69%,41.38%和37.93%;在海洛因组分别为40.74%,33.33%和25.93%,组间差异无显著性P＞0.05）.结论 海洛因依赖改变大鼠海马神经元的痛觉调制,并且以影响背侧海马为主.Objective The heroin-dependent animal model of rats was used to investigate the effects of heroin on regulation of pain perception in the dorsal and ventral hippocampus of the heroin-dependent rats. Meth ods Heroin was injected subcutaneously twice a lay for 9 days according to the principle of daily increasing dose in the Sprague-Dawley rats. From the 10th day,rats were given heroin at dose of 27 mg · kg-1 once a day until the 14th day, then the unit discharges of the dorsal and ventral hippocampus of rats were observed respectively after noxious electric stimulation of the rat-tail by the extracellular single-unit recording with glass microelectrodes. Results When given noxious stimulation, most of the neurons in the dorsal hippocampus in the heroin-dependent rats were unaffected（59.09％ ） ,whereas in the control rats ,the ratio of the neurons of the dorsal hippocampus affected by noxious stimulation was about 66.67％, respectively（P ＜ 0.05 ）. However,in the ventral hippocampus, the ratio of the neurons activated,inhibitory or unaffected was 20. 69％ ,41.38％ and 37.93％ from the control and was 40.74％ ,33. 33％ and 25.93％ from the heroin group respectively with no significant difference between two groups （P ＞ 0.05 ） . Conclusion Heroin changed the regulation of pain perception in the hippocampus,primarily the dorsal hippocampus of rats.

关 键 词：海洛因 海马 单位放电 痛觉 电刺激 

分 类 号：R749.64[医药卫生—神经病学与精神病学]