不同牛群体间钙蛋白酶1基因第14内含子4685bp位点的多态性分析  

Polymorphism Analysis on Intron 14 4685 bp Loci of CAPN1 Gene in Different Cattle Population

作　　者：张立春[1] 胡成华[1] 李赵志[2] 曹阳[1] 周国利[3,4] 金海国[1] 

机构地区：[1]吉林省农业科学院畜牧分院,公主岭136100 [2]延边大学农学院,龙井133002 [3]东北农业大学动物科技学院,哈尔滨150030 [4]聊城大学生命科学学院,聊城252059

出　　处：《中国畜牧兽医》2010年第8期107-111,共5页China Animal Husbandry & Veterinary Medicine

基　　金：优质肉牛杂种优势持续利用技术研究(2007BAD55B02);国家高技术研究发展计划(863)项目(2008AA101010);国家"十一五"科技支撑项目(2008BADB2B01)

摘　　要：本研究采用PCR-RFLP技术分析了钙蛋白酶1(CAPN1)基因第14内含子4685bp位点在东北地区特色养殖品种草原红牛、延边黄牛及不同二元杂交品种红安格斯牛×西门塔尔、利木赞×西门塔尔、德国黄牛×西门塔尔和夏洛莱×西门塔尔等群体间的多态性。结果表明,4685bp位点无论是基因型频率还是等位基因频率,在以上牛群体间均存在较大差异。延边黄牛中CT基因型作为优势基因型其频率达到0.53,CC及TT基因型频率差异不大,分别为0.27和0.20;德国黄牛×西门塔尔二元杂交牛中TT、CT基因型具有非常高的基因型频率,分别达到0.50和0.40,而CC基因型则只有0.10,T等位基因频率高达0.70,C等位基因频率仅为0.30。其他二元杂交品种无论是基因型频率还是等位基因频率上均与延边黄牛相类似。本研究中最值得关注的是草原红牛中4685bp位点仅存在1种基因型,即为TT型,未发现C等位基因的存在。3种牛群体间4685bp位点基因型频率的比较,为进一步分析该位点与肉质嫩度的相关性奠定了基础。CAPN1 gene as the major gene which controls beef tenderization was concerned by researchers. In this study,we reported the 4685 bp loci genotype frequency and allele frequency in Chinese Red Steppe,Yanbian Yellow cattle, Red Angus×Simmental, Limousin×Simmental, Germany Yellow cattle×Simmental and Charolais×Simmental cross. The results showed that genotype frequency and allele frequency were significant different in these population. As the predominant genotype in Yanbian Yellow cattle,the frequency of CT genotype was 0.53,the frequency of CC and TT genotype just were 0.27 and 0.20 respectively. The TT and CT genotype had very high frequency in Germany Yellow cattle×Simmental cross, reached 0.50 and 0.40 respectively, the CC genotype frequency only was 0.10, the T allele frequency reached 0.70, and the C allele frequency only was 0.30. The others cross cattle had similar genotype and allele frequency to Yanbian Yellow cattle. The most interesting result in this study was the genotype distribution in Chinese Red cattle,we only found one genotype TT which suggested C allele was not exist in Chinese Red cattle. The complement of genotype frequency test of 4685 bp loci in CAPN1 gene in triple cattle laid a foundation to research the relationship of 4685 bp loci to meat tenderness.

关 键 词：草原红牛 延边黄牛 杂交牛 CAPN1 PCR-SSCP 

分 类 号：S823.8[农业科学—畜牧学]