重型抑郁症患者亚甲基四氢叶酸还原酶基因多态性检测  

The detection of gene polymorphisms of MTHFR C677T and A1298C in severe depression patients

作　　者：冯磊光[1] 邵春青[2] 刘英慧[1] 陶永红[3] 郝潇蕾[1] 

机构地区：[1]哈尔滨医科大学附属第一医院检验科,黑龙江哈尔滨150001 [2]北京市天坛医院检验科,北京100700 [3]哈尔滨医科大学附属第一医院精神科,黑龙江哈尔滨150001

出　　处：《基础医学与临床》2010年第8期811-814,共4页Basic and Clinical Medicine

基　　金：黑龙江省科技攻关课题(GB06C40107)

摘　　要：目的探讨北方汉族人群5,10-亚甲基四氢叶酸还原酶基因多态性与重型抑郁症的关系。方法采用病例-对照研究。聚合酶链反应-限制性片段长度多态性技术检测MTHFRC677T及A1298C基因多态性。结果 (1)对照组677TT基因型频率及T等位基因频率分别为13.16%和39.80%;1298CC基因型和C等位基因频率分别为1.32%和12.83%;(2)抑郁症组MTHFR677TT基因型频率(35.53%)明显高于正常对照组(13.16%)(P<0.001),677 T等位基因频率(57.24%)明显高于对照组(39.80%)(P<0.001)。(3)Logistic回归分析,C677T基因型与疾病的发生有关(P<0.001)。结论 MTHFRC677T基因变异与本组重症抑郁症发病有关,是其发病的危险因素;MTHFRA1298C基因变异与本组重症抑郁症发病无关联。Objective Investigating the relationships of polymorphisms of 5,10-MTHFR gene and severe depression in northern Chinese Han population to provide evidences for the prevention and cure of diseases.Methods Using case-control study,we detected the gene polymorphisms of MTHFR C677T and A1298C by polymerase chain reaction-restriction fragment length polymorphism（PCR-RFLP）.Results（1）The frequency of 677TT in control groups and that of T alleles were 13.16% and 39.80%;the frequencies of genotypes of 1298CC and that of C alleles were 1.32% and 12.83%;（2）The frequency of MTHFR 677T/T genotype（35.53%） and 677T allele（57.24%） in depression was respectively higher than that in control group（13.16%）（P0.001）;the differences of the frequencies of MTHFR A1298C genotype and C allele between control group were respectively no statistically significant（P0.05）;（3）The Logistic regression analysis showed that the genotype of C677T was relative to the occurrence of disease,and the genotype of A1298C was not relative to the occurrence of disease.Conclusion Themutation of MTHFR C677T in severe depression patients was relative to the occurrence of severe depression disease,and it is the risk factors of disease;the mutation of MTHFR A1298C was not relative to the occurrence of the severe depression.

关 键 词：重型抑郁症 聚合酶链反应-限制性片段长度多态性 5 10-亚甲基四氢叶酸还原酶 基因多态性 

分 类 号：R749.4[医药卫生—神经病学与精神病学]