α-干扰素对血管瘤血管内皮细胞周期、凋亡的影响  

Interferon-a induces apoptosis in vascular endothelial cells in vitro

作　　者：刘鹏[1] 俞松[1] 侯昉[1] 曹江[1] 朱明[1] 吕欣[1] 王凯[1] 

机构地区：[1]遵义医学院附属医院小儿矫形外科,563003

出　　处：《中华小儿外科杂志》2010年第8期575-578,共4页Chinese Journal of Pediatric Surgery

基　　金：贵州省科技厅基金[黔科合J字（2007）2215号];贵州省社发攻关项目[黔科合SY（2008）3028号];遵义市重点科技计划项目培育及人才培养计划项目[遵科培字（2009）04号];贵州省教育厅基金（C-290）及遵议医学院基金（2004）01号

摘　　要：目的 观察α-干扰素对体外培养血管瘤内皮细胞周期、凋亡的影响,探讨干扰素治疗血管瘤的机制.方法 用组织块法培养增生期血管瘤内皮细胞,分别用1×104U,1×105U,1×106Uα-干扰素干预,对照组不加任何药物,继续培养24h后,用流式细胞仪检测细胞周期、凋亡变化.结果 α-干扰素作用24 h后血管瘤内皮细胞G1期前出现二倍体凋亡峰;1×104U、1×105U、1×106Uα-干扰素组G1期百分率分别为（63.68±0.66）%、（72.97±1.56）%、（63.27±5.64）%,对照组为（58.75±0.32）%,与对照组比较,P＜0.05;1×104U、1×105U、1×106Uα-干扰素组S期百分率分别为（32.99±2.01）%、（25.02±1.31）%、（37.93±5.31）%,对照组为（26.46±1.94）%,与对照组比较,P＞0.05;1×104U、1×105U、1×106Uα-干扰素组G2/M期百分率分别为（0.01±0.05）%、（2.43±0.27）%、（3.99±1.27）%,其百分率随α-干扰素浓度升高而增高.细胞凋亡百分率随α-干扰素浓度增加而增高,1×104U、1×105U、1×106Uα-干扰素组凋亡百分率分别为（11.89±0.56）%、（18.88±3.04）%、（31.92±1.92）%,对照组凋亡率为（3.25±0.23）%,与对照组比较,P＜0.01.结论 α-干扰素能导致血管瘤血管内皮细胞G1、G2期阻滞和凋亡,细胞凋亡率与药物浓度存在剂量依赖关系.干扰素可能通过血管内皮细胞G1、G2期阻滞,抑制血管瘤血管内皮细胞的增殖、诱导血管内皮细胞凋亡而达到治疗血管瘤的作用.Objective To study the mechanism of interferon-α in hemangioma treatment. Methods The hemangioma vascular endothelial cells isolated from hemangioma were cultured. The cells were treated with 1×104 U, 1×105 U, 1×106U interferon-α fior 24 hours. The changes of cell cycles and apoptosis were assessed by flow cytometry. Results The percentage of G1 phase cells increased with the increasing concentration of interferon-α, and the hypodiploid apoptosis peak appeared before G1 phase. In the interferon-α treated groups （1×104 U,1×105 U, 1×106 U）, the percentage of G1 phase were （63. 68 ± 0.66） %, （72. 97 ± 1.56） %, （63.27 ± 5.64） %respectively whereas in the control group, it was （58. 75 ± 0. 32） %. The percentages of S phase cells in the treated groups were （32. 99 ±2. 01 ） %, （25.02 ± 1.31 ） %, （37.93 ± 5.31 ） %, respectively whereas in the control group, it was （26.46±1.94） %. The percentage of G2/M phase were （0.01 ± 0.05） %, （2. 43 ± 0. 27） %, （3. 99 ±1.27） %. The percentage of S phase cells in the treated and the control groups showed no statistically significant change （P＞0.05）. The G1, G2/M phase percentage of each group revealed significant differences （P＜0.01）. The apoptosis rates in the treated groups were （11.89 ± 0. 56）%, （18.88 ±3.04） %, （31.92 ± 1.92） % respectively. Compared to the control group of 3.25 ± 0.2） %, the apoptosis rates were increased （P＜0.01 ）. Conclusions The mechanisms of the interferon-α in hemangioma treatment might be related with G1, G2 arrest and apoptosis. The rate of apoptosis increase was dose-dependent.

关 键 词：血管瘤 内皮细胞 细胞培养 干扰素Α 细胞凋亡 细胞周期 

分 类 号：R732.2[医药卫生—肿瘤]