原子力显微镜观察粪肠球菌的超微结构及生物膜的动态形成过程  

Use of atomic force microscopy to observe the surface and the dynamic forming process of biofilms of Enterococcus faecalis

作　　者：颜艳[1] 徐晨彪[2] 牛卫东[1] 

机构地区：[1]大连医科大学口腔医学院口腔内科教研室 [2]大连理工大学物理与光电工程学院,辽宁大连116044

出　　处：《华西口腔医学杂志》2010年第4期447-449,454,共4页West China Journal of Stomatology

基　　金：国家自然科学基金资助项目(30870670)

摘　　要：目的应用原子力显微镜(AFM)观察生理状态下粪肠球菌的表面超微结构及粪肠球菌生物膜的动态形成过程。方法利用AFM观察生理状态下粪肠球菌的超微结构并进行三维成像。利用硝酸纤维素薄膜在体外构建粪肠球菌生物膜模型,用AFM观察粪肠球菌生物膜形成过程中的表面变化。结果粪肠球菌菌体呈球状,表面凹凸不平,被颗粒状物包绕。初步确定了6h粪肠球菌生物膜逐渐形成,24h生物膜维持稳定状态,观察到细菌表面局部特征的变化及细菌胞外的多聚体物质。结论应用AFM能够在生理条件下清晰地观察粪肠球菌表面超微结构及粪肠球菌生物膜的整个动态形成过程。Objective To observe the surface of Enterococcus faecalis and the dynamic forming process of those biofilms using atomic force microscopy（AFM） in air condition.Methods The surface of Enterococcus faecalis which were dried in air were observed with AFM.We used the cellulose nitrate film to construct the Enterococcus faecalis biofilms model in vitro,and then placed the biofilms under AFM to observe the surface changes of biofilms＇ development.Results The cell surfaces of strain Enterococcus faecalis were not regular because of the presence of the amorphous substance on the colony surface,which congregated globular,fibrous structure.Gradually determined that at 6 h the initial biofilm formed and at 24 h the biofilms maintained the steady-state.AFM height images showed topographical changes due to biofilms＇ development,which were used to characterize several aspects of the bacterial surface,such as the presence of extracellular polymeric substance,and the biofilms＇ development stage.Conclusion Application of AFM in physiological conditions could be useful in observing Enterococcus faecalis surface ultrastructure and dynamic process of biofilms formation.

关 键 词：粪肠球菌 生物膜 原子力显微镜 

分 类 号：R781.05[医药卫生—口腔医学]