串联型黄色荧光蛋白表达载体的构建及其在柔嫩艾美球虫中的瞬时表达  

Construction of tandem yellow fluorescent protein expression vector and its transient expression in Eimeria tenella

作　　者：李继东[1,2] 郝力力[2] 刘贤勇 周晓艳[2] 索勋[2] 

机构地区：[1]宁夏大学农学院,宁夏银川750021 [2]中国农业大学动物医学院,北京100193

出　　处：《中国兽医科学》2010年第1期11-14,共4页Chinese Veterinary Science

基　　金：国家自然科学基金项目(30471298)

摘　　要：为了探索黄色荧光蛋白(YFP)在柔嫩艾美球虫子孢子中的瞬时表达情况,以柔嫩艾美球虫的组蛋白4(Histone4,H4)上游启动序列和肌动蛋白(Actin)下游调控序列为元件构建了串联型YFP重组转染载体pH4-YFP-YFP-ACT。将构建的载体经电穿孔法转染柔嫩艾美球虫子孢子,并在MDBK细胞中进行培养。结果显示,在荧光显微镜下可以观察到黄色荧光。提示,串联型YFP可以在柔嫩艾美球虫中瞬时表达。To explore the transient expression of yellow fluorescent protein（YFP） in Eimeria tenella sporozoites,an eukaryotic vector pH4-YFP-YFP-ACT, which contains tandem repeated YFP, upstream flanking sequence of Histone4（H4） and downstream regulatory sequence of Actin both derived from E. tenella genomic DNA,was constructed. The sporozoites of E. tenella were electroporated with this expres-sion vector and then cultured in MDBK cells lines. During the course of parasite development,YFP within parasites was observed under fluorescence microscope,indicating that the tandem YFP genes could be ex-pressed in E. tenella.

关 键 词：柔嫩艾美球虫 黄色荧光蛋白 瞬时转染 

分 类 号：S852.723[农业科学—基础兽医学]