甲异靛对K562细胞凋亡的影响  

Apoptosis Inducing Effect of Meisoindigo on K562 Cells

作　　者：宋鲁燕[1] 钱林生[1] 

机构地区：[1]中国医学科学院中国协和医科大学血液学研究所血液病医院

出　　处：《中国中西医结合杂志》1999年第6期353-355,共3页Chinese Journal of Integrated Traditional and Western Medicine

基　　金：卫生部课题基金

摘　　要：目的：研究甲异靛对人慢性粒细胞白血病细胞系Ｋ５６２细胞的影响，探讨甲异靛治疗慢性粒细胞白血病（ＣＭＬ）的作用机理。方法：应用剂量反应曲线，台盼蓝拒染实验，细胞形态学，ＤＮＡ电泳，流式细胞仪及ＴＵＮＥＬ标记等多种方法观察甲异靛对Ｋ５６２细胞的作用。结果：甲异靛能明显抑制Ｋ５６２细胞的增殖，并同时诱导Ｋ５６２细胞凋亡。结论：甲异靛治疗ＣＭＬ的机理可能与甲异靛引起白血病细胞受抑和细胞发生凋亡有关。Objective: To study the effect of meisoindigo on human chronic myelogenous leukemia (CML) cell line K562 cells for exploring the mechanism of meisoindigo in treatment of CML. Methods: Multiple methods, including dose response curve, trypan blue exclusion, cytomorphology, DNA electrophoresis, flow cytometry and TUNEL (TdT mediated dUTP nick and labeling), were used to observe the effect of meisoindigo on K562 cells. Results: Meisoindigo can inhibit the proliferation and induce the apoptosis of K562 cells. Conclusion: The therapeutic mechanism of meisoindigo in treating CML may be related with its proliferation inhibiting and apoptosis inducing effect in CML cells.

关 键 词：甲异靛 慢性 白血病 细胞凋亡 

分 类 号：R733.720.2[医药卫生—肿瘤]