高效液相色谱法测定甘草黄酮阿魏酸乳膏中阿魏酸含量  

Content Determination of Ferulic Acid in Glycyrrhiza Flavonoid and Ferulic Acid Cream by High Performance Liquid Chromatography

作　　者：谢军[1,2] 张典瑞[1] 刘圣[2] 江晓[3] 

机构地区：[1]山东大学药学院,山东济南250012 [2]安徽省立医院药剂科,安徽合肥230001 [3]安徽中医学院药学院,安徽合肥230038

出　　处：《安徽中医学院学报》2010年第4期68-70,共3页Journal of Anhui Traditional Chinese Medical College

摘　　要：目的建立甘草黄酮阿魏酸乳膏中阿魏酸含量的测定方法。方法采用KromasilC18（4．6mm×250mm，5μm）色谱柱；流动相为乙腈-0．3％冰醋酸（25：75）；流速为1．0ml／min；柱温25℃；检测波长320nm；进样量20μl。结果阿魏酸浓度为2．26-36．16μg／ml时与峰面积呈良好的线性关系（r=0．9998），平均加样回收率为97．50％，RSD为1．73％（n=6）。结论本方法准确、简便、重现性好，可作为该品种中阿魏酸的定量检测方法。Objective To eastablish a method to determine ferulie acid in Glycyrrhiza Flavonoid and Ferulic Acid Cream. Methods Kromasil C18 column（4. 6 mm×250mm,5μm）was used. The chromatographic conditions were as follows： the mobile phase was acetonitrile 0.3% glacial acetic acid （25： 75）; the detection wavelength was set at 320 nm; the column temperature was at 25℃ ; the flow rate was 1.0 ml/min; the sampling size was 20 μl. Results There was a linear relationship between the concentration of ferulic acid and the peak area with the range of 2.26 to 36.16/,g/ml （r=0. 999 8）. The average recovery rate was 97.50%, and RSD was 1.73% （n=6）. Conclusion This method can be used for content determination of ferulic acid in this preparation because of its accuracy, high sensitivity and good reproducibility.

关 键 词：甘草黄酮阿魏酸乳膏 阿魏酸 高效液相色谱法 含量测定 

分 类 号：R927.2[医药卫生—药学]