盐酸利多卡因体外对精子作用的扫描电镜观察  

Scanning electronic microscope observation of the action of lidocaine hydrochloric acid to human sperm in vitro

作　　者：丁贤胜 高晓勤[2] 倪佳[3] 

机构地区：[1]六盘水市人民医院病理科,贵州六盘水553001 [2]遵义医药高等专科学校,贵州遵义563002 [3]贵阳医学院组织胚胎学教研室,贵州贵阳550004

出　　处：《检验医学》2010年第8期621-624,共4页Laboratory Medicine

基　　金：贵州省省长专项基金资助项目[黔科教办(2007)03];贵州省科技厅攻关项目[黔科合S字(2007)1030]

摘　　要：目的探讨盐酸利多卡因（LHA）体外对人精子形态结构改变的机制。方法选择健康生育男性8名,年龄23～32岁,禁欲3～7d后,手淫取精。分为对照组（A组）,实验B组（LHA终浓度为5mg/mL）、实验C组（LHA终浓度为15mg/mL）。体外孵育1、2h后,采用S3400N扫描电镜观察各组精子形态的改变。结果与A组相比,实验B组1h后精子形态结构改变差异无统计学意义（P〉0.05）,2h后差异有统计学意义（P〈0.05）;实验C组1h后差异有统计学意义（P〈0.05）,2h后差异更为明显（P〈0.01）。结论随着LHA终浓度的增加和孵育时间的延长,LHA对精子形态结构改变越来越明显。Objective To investigate the mechanism of the change of sperm shape after using lidocaine hydrochloric acid （ LHA） in vitro. Methods 8 healthy males,whose age was 23-32 years old,and abstinence 3-7 d,were enrolled in the study. The sperm was collected through the masturbation. The specimens were classified into control group A and experiment group B,C. The LHA were added to the experiment groups respectively at the different concentrations. The concentrations were 5 mg/mL and 15 mg/mL respectively. After fostering 1 h and 2 h in vitro,the changes of sperm shape were examined in the group A ,B and C through the scanning electronic microscope S3400N. Results Compared with group A,group B did not show obvious difference after fostering 1 h（ P〈0. 05） ,but the difference was remarkable after fostering 2 h（ P〈0. 05）. Group C showed difference after fostering 1 h（ P〈0. 05） ,and more remarkable difference after fostering 2 h （ P〈0. 01）. Conclusions With increasing end concentration and extended time,the influence of LHA on the change of sperm shape is more obvious.

关 键 词：盐酸利多卡因 辅助生殖技术 精子 体外杀精剂 扫描电镜 

分 类 号：R446.11[医药卫生—诊断学]