胃癌组织中nm23的表达及其亚细胞组分中核苷二磷酸激酶活力的比较研究  

Expression of nm23 and nucleoside diphosphokinase activity in gastric carcinoma

作　　者：韩颖[1] 王宏路[1] 尹宗柱[1] 金海玲[1] 

机构地区：[1]延边大学医学院生物化学教研室,吉林延吉133000

出　　处：《延边大学医学学报》1999年第2期79-83,共5页Journal of Medical Science Yanbian University

摘　　要：［ 目的］ 揭示胃癌组织及相应癌旁正常组织中ｎｍ２３ 的表达及其与核苷二磷酸激酶活力的相关关系．［ 方法］ 参考了Ｍａｒｉａ Ｋｅｚｄｉ 所报道的核苷二磷酸激酶活性测定方法，应用尹宗柱等所报道的亚细胞结构分离方法分离并测定了２０ 例胃癌组织（ 转移３ 例，未转移１７ 例） 及其对应癌旁正常组织中组织匀浆液、线粒体、微粒体及细胞胞浆中核苷二磷酸激酶的酶活力，同时应用免疫组织化学法观察了ｎｍ２３ 表达阳性率．［ 结果］２０ 例胃癌原发灶中ｎｍ２３ 阳性表达率为４０ ％ ，２０ 例癌旁正常组织中阳性表达率为１０ ％ ，经卡方检验表明二者有显著性差异．胃癌组织匀浆液及胞浆中核苷二磷酸激酶活力（ 每克组织）与比活力（２６５－９９ ±８４－８１ｍ Ｕ，２－７０ ±０－９５ｍ Ｕ／ｍｇ ｐｒｏｔｅｉｎ） ；（２９０－８１±８８－１８ｍ Ｕ，３－５７ ±１－１０ｍ Ｕ／ｍｇ ｐｒｏｔｅｉｎ） 均显著高于相应癌旁正常组织的酶活力及比活力（１８１－２８±６３－６１ ，２２９－５７ ±６４－６７ｍ Ｕ；１－７５ ±０－６１ ，２－７４ ±０－７５ｍ Ｕ／ｍｇ ｐｒｏｔｅｉｎ） ；两组线粒体酶活力及比活力无显著性差异；而在微粒体中癌旁正常组织核苷二磷酸激酶活力及比活力（８?OBJECTIVE To discover the corelationship between nm23 expression and nucleoside diphosphokinase activity in gastric carcinoma.METHOD Homogenate,cytosol,microsome,mitochondria were obtained from gastric carcinoma and normal tissues and nucleoside diphosphokinase activity was determined by Kezdi's method. nm23 expression in those tissues was also studied by using nm23 H 1 monoclonal antibody with avidin biotin complex method. RESULT Nucleoside diphosphokinase activity(per gram tissue) and specific activity in both homogenate, in cancer group(265 99±84 81mU,2 70±0 95mU/mg protein) and cytosol(290 81±88 18mU,3 57±1 10mU/mg protein) are significently higher than those in normal group(181 28±63 61,229 57±64 67mU; 1 75±0 61,2 74±0 75mU/mg protein) Activities and specific activities in mitochondtia of both group has no statistical difference and in microsome fractions in normal group(8 89±5 84mU,1 92±0 88mU/mg protein) are a little higher than those in cancer group(5 70±2 60mU,1 37±0 61mU/mg protein). CONCLUSION The nucleoside diphosphokinase activity in nonmetastatic tissue is higher than that in normal tissue.

关 键 词：核苷二磷酸激酶 胃肿瘤 NM23 病理 癌基因 

分 类 号：R735.202[医药卫生—肿瘤]