曲酸高产菌株的诱变选育  

Mutagenesis of Strain with High Yield of Kojic Acid

作　　者：赵鑫[1,2] 赵金[1] 吴思国[1,2] 胡翠敏[1,2] 赵宗保[1] 

机构地区：[1]中国科学院大连化学物理研究所,辽宁大连116023 [2]中国科学院研究生院,北京100039

出　　处：《激光生物学报》2010年第4期542-545,共4页Acta Laser Biology Sinica

基　　金：中国科学院知识创新工程领域前沿项目(S200719)

摘　　要：以紫外线和氮离子注入作为诱变手段对曲酸产生菌黄曲霉CICC 2242进行诱变处理,筛选出一株高产菌株N83。曲酸产量由初始的12.4g/L提高到19.4g/L,提高了56.5%;遗传稳定性实验结果表明,N83遗传性状稳定良好。该结果表明氮离子注入诱变是获得高产曲酸的有效途径。The kojic acid-producing strain Aspergillus flavus CICC 2 242 was mutagenized using UV radiation and N^＋ implantation, and its mutant strain A. flavlts N83 was obtained. The mutant strain produced kojic acid at 19.4 g/L, which was 56.5 % higher than that with A. flavus CICC 2 242 at 12.4 g/L. Moreover, A. flavus N83 showed good genetic stability. The results showed that N^＋ implantation was an effective method to generate better kojic acid producer.

关 键 词：曲酸 紫外线 氮离子注入 诱变 

分 类 号：Q631[生物学—生物物理学] Q813