口腔癌组织中缺氧微环境对单核/巨噬细胞分泌VEGF影响的实验研究  

Experimental study on secretion of VEGF of monocytes/macrophages in hypoxic microenvironment in oral cancer

作　　者：冯红超[1] 彭江帆[1] 马洪[1] 宋宇峰[1] 

机构地区：[1]贵阳医学院口腔医学系口腔颌面外科教研室,贵阳550004

出　　处：《重庆医学》2010年第17期2244-2245,共2页Chongqing medicine

基　　金：国家自然科学基金资助项目(30660200);贵州省优秀科技教育人才省长专项基金资助项目(2005)160号

摘　　要：目的通过缺氧微环境对单核/巨噬细胞分泌血管内皮生长因子(VEGF)影响的实验研究,探讨口腔癌组织局部微环境对单核/巨噬细胞功能的影响。方法从健康人的外周血中分离单个核细胞并通过培养将其转化为单核/巨噬细胞;分别在缺氧(O2<1%)和常氧条件(20%O2)下加入口腔鳞癌细胞系Tca8113培养上清液进行单核/巨噬细胞培养,在3、5、7 h采用酶联免疫吸附试验(ELISA)法检测培养上清液中VEGF的表达。结果在缺氧微环境下,单核/巨噬细胞分泌VEGF的能力较常氧环境下增加,并随时间的延长而增加(P<0.05)。结论在缺氧的微环境下,单核/巨噬细胞可能更多的通过生长因子的分泌参与肿瘤的生长,但单核/巨噬细胞在肿瘤组织中的作用其具体机制及在肿瘤免疫治疗中的意义还需进一步的研究。Objective To investigate the secretion of vascular endothelial growth factor（VEGF） of monocytes/macrophages in hypoxic microenvironment of oral cancer in vitro.Methods Peripheral blood mononuclear cells were extracted from healthy person′s blood and cultivated to transform into monocytes/macrophages.Then monocytes/macrophages were cultured in hypoxic microenvironment（O〈21%） and in normal microenvironment（20% O2）.The expression of VEGF was detected by enzyme-link immunoassay（ELISA）.Results The expression of VEGF excreted by mononuclear/macrophage was significantly higher in hypoxic microenvironment than in normal microenvironment.Conclusion In hypoxic microenvironment,the secretion of VEGF by mononuclear/macrophage is gradually increased.But the mechanism of monocytes/macrophages′ function in tumor needs more research.

关 键 词：单核/巨噬细胞 口腔癌 缺氧 血管内皮生长因子 

分 类 号：R739.85[医药卫生—肿瘤] R733[医药卫生—临床医学]