老年和成年大鼠术后认知功能障碍模型的建立与比较  

Establishment and evaluation of model of postoperative cognitive dysfunction in adult and aged rats

作　　者：曹剑[1] 李鹏[1] 易斌[1] 罗铁山[1] 石胜驰[1] 陶国才[1] 

机构地区：[1]第三军医大学西南医院麻醉科,重庆400038

出　　处：《重庆医学》2010年第17期2292-2293,2296,共3页Chongqing medicine

摘　　要：目的建立不同月龄大鼠麻醉术后认知功能障碍(POCD)模型并进行比较,为POCD机制研究提供模型选择。方法以4月龄和20月龄SD雄性大鼠为研究对象,分别接受异氟醚麻醉处理和腹腔探查手术2 h,建立吸入麻醉和手术应激模型。术后分别进行Morris水迷宫、穿梭箱实验检测大鼠行为学变化。结果老年大鼠在接受麻醉和手术后出现不同程度的认知能力下降,并且以手术组最为明显,持续时间较长;成年大鼠接受手术后出现行为学改变,但程度较轻,1周后恢复,而成年麻醉组大鼠未见明显行为学改变。结论老年大鼠手术后认知功能损害明显,可作为POCD研究的动物模型。Objective To establish and assess adult and aged rats model of postoperative cognitive dysfunction（POCD） in order to provide the model for researching POCD pathogenesis.Methods Adult and aged rats were randomly divided into six groups with 10 rats in each group.Group I1 and I2 were anesthetized with 1.5% isoflurane for 2 h;during the inhalation anaesthesia with 1.5% isoflurane,the rats in group O1 and group O2 accepted abdominal operation for 2 h;the learning and memory function was assessed using Morris water-maze test and Shuttle box trial after anaesthesia and operation.Results The result of the tests showed that there were different degrees of cognitive decline in the aged rat after anesthesia and surgery but the change was more obvious and longer in the O1 group which received operation.In the adult rats,there was behavioral change last for a week after surgery while no significant behavioral changes were fuond in the adult anesthesia group.Conclusion Isoflurane anesthesia and exploratory abdominal surgery result in a different month-old rats varying degrees of cognitive impairment,particularly in old rats after receiving surgery and it can be used as postoperative cognitive dysfunction animal model for pathogenesis study.

关 键 词：大鼠 异氟醚 认知 模型 手术后并发症 

分 类 号：R749.2[医药卫生—神经病学与精神病学]