nm23H1正反义表达载体的构建  

Construction of expression vector of sense and antisense nm23H1

作　　者：金嗣松[1,2] 潘星华[1,2] 王元和[1,2] 胡以平[1,2] 傅继梁[1,2] 

机构地区：[1]第二军医大学基础医学部生物学教研室 [2]第二军医大学长征医院普通外科

出　　处：《第二军医大学学报》1999年第6期350-352,共3页Academic Journal of Second Military Medical University

基　　金：国家自然科学基金

摘　　要：目的：构建ｎｍ２３Ｈ１正反义表达载体ｐｃ３ＡＮ和ｐｃ３ＡＭ。方法：将ｎｍ２３Ｈ１基因正、反向插入质粒ｐｃＤＮＡ３和ｐＲＣ／ＣＭＶ，并转染肝癌细胞ＳＭＭＣ７７２１。结果：转染正义表达载体后ｎｍ２３Ｈ１ｍＲＮＡ和蛋白表达水平增加，转染反义表达载体后ｎｍ２３Ｈ１ｍＲＮＡ和蛋白表达水平降低。结论：构建的载体能在肝癌细胞内有效表达。Objective: To construct expression vector of sense and antisense nm23H1: pc3AN and pc3AM. Methods: nm23H1 gene was inserted into plasmid pcDNA3 and pRC/CMV in proper sequence and in reverse sequence, the production of above action was used to transfect liver cancer cell SMMC7721. Results: The level of mRNA and protein of nm23H1 was high when transfected with sense vector, and was low when transfected with antisense vector. Conclusion: The constructed vectors can highly express in liver cancer cell.

关 键 词：肝肿瘤 NM23H1基因 正反义表达载体 载体构建 

分 类 号：R735.702[医药卫生—肿瘤] Q78[医药卫生—临床医学]