伯氏疟原虫小亚单位rRNA在斯氏按蚊体内的转录类型和动态  

Transcription types and dynamics of Plasmodium berghei SSUrRNA in Anopheles stephensi

作　　者：刘延刚[1] 宋关鸿[1] 徐建农[1] 

机构地区：[1]第二军医大学基础医学部寄生虫学教研室

出　　处：《第二军医大学学报》1999年第6期368-370,共3页Academic Journal of Second Military Medical University

基　　金：国家自然科学基金

摘　　要：目的：观察伯氏疟原虫（Ｐ．ｂｅｒｇｈｅｉ）小亚单位核糖体ＲＮＡ（ＳＳＵｒＲＮＡ）在斯氏按蚊体内的转录类型和动态。方法：应用ＲＴ－ＰＣＲ方法，特异地扩增伯氏疟原虫Ａ型和Ｓ型ＳＳＵｒＲＮＡ，检测斯氏按蚊虫体内Ｓ型ＳＳＵｒＲＮＡ的转录水平。结果：吸感染血的斯氏按蚊体内仅见Ｓ型ＳＳＵｒＲＮＡ的条带。整体检测从第２天开始查见，第８天明显增强，第１２～１６天达到高峰。在蚊腹部Ｓ型ＳＳＵｒＲＮＡ转录动态与整体检测结果相似。胸部于第１２天查见该基因的转录。结论：斯氏按蚊体内伯氏疟原虫仅转录Ｓ型ＳＳＵｒＲＮＡ，它的转录动态与疟原虫在蚊体内的发育密切相关。Objective: To detect and observe types and transcription dynamics of small subunit ribosomal RNA (SSUrRNA) of Plasmodium berghei in Anopheles stephensi. Methods: The A and Stype SSU rRNA of the parasites were distinctively amplyfied by RTPCR. The transcription levels of the StypeSSUrRNA in different parts of the mosquitoes were detected. Results: Only the Stype SSUrRNA was detected in the parasiteinfected mosquitoes. The Stype SSUrRNA electrophoresis band was seen on second day after engorgment of the mosquitoes. On d 8, its intensity increased remarkably and reached the peak on d 1216. The transcription dynamics of the Stype SSUrRNA in the mosquito abdomen were similar to those of the whole mosquitoes. In contrast, the transcription of the SSUrRNA was detected in the mosquito chest on d 12. Conclusion: Only the Stype SSUrRNA of the P.berghei is transcribed during their sporogony development. The transcription dynamics of the SSUrRNA may be responsible for the sporogony development of the p

关 键 词：伯氏疟原虫 小亚单位 RRNA 斯氏按蚊 

分 类 号：R382.31[医药卫生—医学寄生虫学] R384.1[医药卫生—基础医学]