505例白血病应用流式细胞术免疫分型与FAB分型的结果分析  

Analysis of the result of Immunophenotype by Flow Cytometry and FAB phenotype of 505 cases of leukemia

作　　者：杨峥[1] 莫武宁[1] 李山[1] 秦雪[1] 兰会华[1] 陈正立[1] 

机构地区：[1]广西医科大学第一附属医院临床医学实验部,广西南宁530021

出　　处：《中国实验诊断学》2010年第8期1238-1241,共4页Chinese Journal of Laboratory Diagnosis

摘　　要：目的探讨白血病免疫分型与FAB分型的关系,以指导临床进行分析与治疗。方法对505例白血病患者分别进行细胞形态学检查和流式细胞术(FCM)免疫分型,并对2种检查结果进行分析。结果①505例白血病患者中FAB初诊急性髓细胞白血病(AML)248例,急性淋巴细胞白血病(ALL)200例,慢性粒细胞白血病(CML)18例,慢性淋巴细胞白血病(CLL)15例,混合型白血病(HAL)14例,少见类型10例;免疫分型初诊单纯型AML163例,变异型AML83例,单纯型ALL108例,变异型ALL106例,双表型32例,未分化型13例。②AML形态学分型与免疫分型完全符合率61.3%、部分符合率30.2%;ALL形态学分型与免疫分型完全符合率53%,部分符合率44.5%;混合型符合率42.9%。结论免疫分型在鉴别AML与ALL各亚型和变异型及混合型白血病方面具有重要作用,免疫分型比形态学分型结果更为精细、准确,是形态学分型的补充与修正,但不能取代形态学分型,二者结合可提高诊断准确率。Objective To study the relationship of immunophenotype and FAB phenotype and guide clinical analysis and treatment. Methods Cell morphology checking and immunophenotyping were performed at the same time for the 505 patients with leukemia, flow cytometry had used for immunophenotype, on the end the results had been analyzed to understand the relatiomhip between the two phenotypes. Results OAmong the 505 cases of leukemia, there were AML（248） ,ALL（200） ,CML（18） ,CLL（ 15）, HAL（14） ,UAL（10） in FAB phenotype and AML（163） ,Ly ＋ AML（83） ,ALL（108）, My ＋ ALL（ 106）, HAL（32） ,UAL（13） in im- munophenotype. 0 In AML the accordance rate and part accordance rate of the two phenotypes was 61.3 % and 30.2%. In ALL the accordance rate was and part accordance rate was 53 % and 44.5 %. In HAL the accordance rate was 42.9 %. Conclusion Immunophenotype plays an important role to distinguish AML, ALL subtype, variants leukemia and HAL. Immunophenotyping was shaper and more accurate than FAB phenotype, and is a supplementary and correction, but it can not replace the FAB phenotype. Combination of both can improve the diagnostic accuracy.

关 键 词：白血病 免疫分型 形态学分型 

分 类 号：R733.7[医药卫生—肿瘤]