复方中药制剂对感染根管常见细菌生物膜的作用  

Effect of compound Chinese traditional medicine on infected root canal bacteria biofilm

作　　者：马瑞[1] 黄莉莉[1] 夏文薇[1] 朱彩莲[2] 叶冬霞[2] 

机构地区：[1]上海交通大学医学院附属第九人民医院.口腔医学院牙体牙髓病科,上海200011 [2]上海市口腔医学重点实验室,上海200011

出　　处：《上海口腔医学》2010年第4期415-418,共4页Shanghai Journal of Stomatology

基　　金：上海市卫生局中医处资助项目(2008L008A)~~

摘　　要：目的:观察由没食子酸、厚朴酚及白芨多糖三者配伍制成的复方中药制剂对感染根管常见细菌混合生物膜的抗菌作用。方法:选择感染根管内常见细菌黏性放线菌、具核梭杆菌、粪肠球菌,在体外形成24h混合细菌生物膜,分别作用于复方中药制剂和氧化锌溶液30min后,应用激光共聚焦扫描显微镜观察生物膜厚度和细菌活性的变化,采用SAS6.12软件包中的秩和检验进行统计学分析。结果:复方中药制剂、氧化锌溶液作用后,生物膜厚度均下降,两者间无显著差别(P>0.05);使用中药制剂后,生物膜活菌百分比显著下降,强于氧化锌溶液,有显著性差异(P<0.01)。结论:复方中药制剂对感染根管内常见细菌混合生物膜具有较强的抑制作用,将其作为根管封闭剂中的抗菌成分,具有一定的应用前景。PURPOSE： To assess the efficacy of compound Chinese traditional medicine（CTM）,which composed of gallic acid,magnolol and polysaccharide of Blettila striata,against the infected root canal bacterial biofilm.METHODS： Actinomyces viscosus（Av）,Enterococcus faecalis（Ef）,Fusobacterium nucleatum（Fn） were composed to form biofilm,then confocal laser scan microscope（CLSM） was used to observe and study the bacterial activity.SAS6.12 software package was used for statistical analysis.RESULTS： The biofilm thickness reduced after treatment by both CTM and ZnO（P〈0.05）,while there was a significant decrease of the percentage of vital bacterias after treatment by CTM（P〈0.01）.CONCLUSIONS： The compound Chinese traditional medicine is effective on biofilm control,so that it would be an effective disinfecting drug for root canal sealers.

关 键 词：复方中药 具核梭杆菌 黏性放线菌 粪肠球菌 生物膜 根管封闭剂 

分 类 号：R781.05[医药卫生—口腔医学]