大鼠切牙发育中釉蛋白的免疫荧光定位  

Localization of enamelin in developing rat incisor by immunofluorescence

作　　者：刘天麟[1] 王强[2] 

机构地区：[1]同济大学附属东方医院口腔科,上海200120 [2]同济大学附属口腔医院口腔内科,上海200072

出　　处：《上海口腔医学》2010年第4期419-422,共4页Shanghai Journal of Stomatology

基　　金：同济大学"十五""211工程"建设项目(1504102011)~~

摘　　要：目的:观察釉蛋白在大鼠切牙发育不同时期的表达和分布情况。方法:采用免疫荧光法观察釉蛋白在大鼠切牙发育不同时期的表达与分布。结果:在成釉器增殖期、分化早期,未见釉蛋白表达;在分化晚期,前成釉细胞有弱阳性表达;在成熟早期,成釉细胞和成牙本质细胞均阳性表达;在成熟中期,成釉细胞及其基质呈强阳性表达,而成牙本质细胞阳性表达;在成熟晚期,成釉细胞和成牙本质细胞弱阳性表达。在成釉器各期,釉蛋白在外釉细胞、星网状细胞、牙乳头细胞均呈阴性表达。结论:釉蛋白参与釉基质和牙本质基质的形成,可能与牙发育中基质形成的信息传递有关。PURPOSE： To investigate the expression of enamelin in developing rat incisor.METHODS： Immunofluorescence staining for the specimens of developing rat incisor was used to localize the expression of enamelin.RESULTS： Enamelin was not detected at the proliferation phase,and early differentiation phase in preameloblasts.At the advanced differentiation phase,enamelin expressed weakly in ameloblasts and odotoblasts.At the early maturation phase enamelin was detected in ameloblasts and odotoblasts.Enamelin expressed strongly in ameloblasts and enamel matrix but strongly in odotoblasts at the intermediate maturation phase and weakly at the advanced maturation phase.Enamelin was not found in the other stages and position.CONCLUSION： Enamelin,involved in enamel and dentin matrix formation,may play an important role in signal transduction of matrix formation.

关 键 词：釉蛋白 切牙 大鼠 免疫荧光 

分 类 号：R780.2[医药卫生—口腔医学]