外周血淋巴细胞染色体、微核同步制作技术探讨  

STUDY ON SYNCHRONIZATION MANUFACTURE TECHNOLOGY OF PERIPHERAL BLOOD LYMPHOCYTE MICRONUCLEUS AND CHROMOSOME

作　　者：陈刚[1] 刘士云 白玉萍[1] 范雪云[1] 姚三巧[1] 

机构地区：[1]河北省煤矿卫生与安全实验室华北煤炭医学院预防医学系,唐山063000 [2]北戴河煤矿工人疗养院

出　　处：《现代预防医学》2010年第17期3216-3218,共3页Modern Preventive Medicine

摘　　要：[目的]建立人体外周血淋巴细胞染色体及微核同步制作方法。[方法]采用微量全血培养法检测60名X射线接触者外周血淋巴细胞染色体和微核,观察不同浓度的秋水仙素作用不同时间后,淋巴细胞微核检出率和中期分裂相获得情况。[结果]终止培养前8h加入终浓度为0.05、0.10、0.20、0.40μg/ml的秋水仙素后,不同浓度组间染色体获得率和微核检出率差异无统计学意义;0.1～0.2μg/ml的秋水仙素作用6～10h能够获得易于观察的染色体,同时便于进行微核计数。[结论]在终止培养前6～10h加入0.1～0.2μg/ml的秋水仙素是该同步制作技术的最佳优化条件。[Objective] To established the method for human body peripheral blood lymphocyte micronucleus and chromosome synchronization manufacture. [Methods] 60 workers exposed to X-ray were chosen to test their chromosome and micronucleus of peripheral blood lymphocytes with whole blood culture, and to observe detection rate on micronucleus and metakinesis after different concentrations colchicin at different time. [Results] There was no significant difference in pick-up rates of chromosome and detection rates of micronucleus in different concentration groups after adding 0.05, 0.10, 0.20, 0.40 g / ml colchicin 8 hours before ending the cultivation, the opportune response time was 6-10 hours to examine the chromosome and micronucleus rate. [Conclusion] The best experimental condition is that adding 0.1-0.2 g / ml colchicin 6-10 hours before ending cultivation for the synchronized manufacture technology.

关 键 词：淋巴细胞微核 染色体 同步制作 

分 类 号：R-33[医药卫生]