抗核周因子在胆道塑料内支架再阻塞中表达作用的研究  

The expression of anti-perinuclear factor in obstructive mechanism of biliary plastic stent

作　　者：吕文[1] 王霞[1] 黄平[1] 黄海涛[1] 范震[1] 张筱凤[1] 张啸[1] 

机构地区：[1]浙江省杭州市第一人民医院,浙江杭州310006

出　　处：《现代实用医学》2010年第7期741-743,共3页Modern Practical Medicine

基　　金：浙江省医药卫生科研基金B类项目;编号:2005B131

摘　　要：目的通过胆道内支架再阻塞内容物的生物化学研究,来进一步探讨胆道内支架阻塞机制。方法收集胆道疾病患者十二指肠内引流术(ERBD)后塑料内支架,肉眼分为支架阻塞组(20例)及未阻塞组(48例)。进行支架内膜抗核周因子(APF)的定量分析、细菌学和胆固醇、结晶钙盐红外透光率分析。结果阻塞组和非阻塞组APF蛋白定量分析分别为(10.39±2.17)和(17.29±9.52)ng/mL,两组差异有统计学意义(<0.05)。阻塞组支架内生物膜的胆红素钙和胆固醇含量均明显高于未阻塞组(<0.01)。与未阻塞的支架相比,大多阻塞的支架内含多种类的细菌。结论阻塞支架内生物膜的主要成份是胆红素钙和胆固醇。APF有助于减缓胆道支架蛋白生物膜的形成,从而保持胆道通畅。Objective To investigate the obstructive mechanism of plastic biliary stents,and to evaluate preliminarily the factors that might impact the formation of biliary stones.Methods All bile cultures collected from patients with plastic biliary stents,identified by gross observation.20 specimens were obstructed（obstructed group）and 48 specimens were not obstructed（unobstructed group）.The anti-perinuclear factor（APF）,bacterium infection,calcium bilirubin and cholesterin were analyzed by biochemistry,and the inner surface of the stents was measured and compared between two groups.Results The total of 68 specimens was collected from patients with plastic biliary stents.The protein level of APF was（10.39±2.17）ng/mL in obstructed group and（17.29±2.52）ng/mL in unobstructed group,and there was a significant difference between groups（P〈0.05）.Calcium bilirubin and cholesterin were the major elements in obstructed group.Many types of bacteria were found in obstructed group.Infected biliary tract was caused by bacterial retrograde.Conclusions Calcium bilirubin and cholesterin were the major elements in obstructed biofilm of the stent,and APF participated in lessening the biofilm formation and smoothing the stent surface.

关 键 词：胆固醇 胆红素钙 抗核周因子 经内镜逆行胰胆管造影 塑料内支架 

分 类 号：R575[医药卫生—消化系统] R576[医药卫生—内科学]