吡格列酮、TNF-α对3T3-L1细胞Perilipin mRNA表达影响的研究  

Effect of perilipin mRNA expression in 3T3-L1 cells by pioglitazone and TNF-α

作　　者：丁振兴[1] 王佑民[2] 张泓[1] 

机构地区：[1]安徽医科大学第一附属医院急诊内科,安徽合肥230022 [2]安徽医科大学第一附属医院内分泌科,安徽合肥230022

出　　处：《安徽医药》2010年第9期1007-1009,共3页Anhui Medical and Pharmaceutical Journal

基　　金：安徽省自然科学基金项目(No03043719)

摘　　要：目的探讨吡格列酮和TNF-α对3T3-L1细胞中perilipin(周脂素)mRNA表达的影响及其时间效应。方法用吡格列酮(100μmol.L-1)和TNF-α(100μg.L-1)分别处理未分化、分化过程中和分化成熟后的3T3-L1细胞,RT-PCR检测不同干预时间perilipin mRNA的表达水平。结果 (1)未分化3T3-L1细胞中perilipin未见表达,分化过程中细胞perilipin mRNA的表达水平随时间递增。(2)在分化过程中和分化成熟的3T3-L1细胞中,吡格列酮都明显增加perilipin mRNA的表达,TNF-α则明显抑制perilipin mRNA的表达。(3)吡格列酮能抵抗TNF-α对成熟3T3-L1脂肪细胞perilipin mRNA表达的抑制作用。结论随着分化成熟,3T3-L1细胞perilipin mRNA表达量逐渐增加;吡格列酮能明显增加3T3-L1细胞的perilipin mRNA表达,TNF-α则明显抑制其表达;吡格列酮能抵抗TNF-α对3T3-L1脂肪细胞perilipin mRNA表达的抑制作用。Aim To observe effect of pioglitazone and TNF-α on perilipin mRNA expression in 3T3-L1 cells and their time-effects.Methods After the 3T3-L1 preadipocytes,differentiating 3T3-L1 cells and differentiated 3T3-L1 adipocytes incubated with pioglitazone（100 μmol·L^-1）,TNF-α（100 μg·L^-1） for different times,cells were collected,total RNA as extracted.The perilipin mRNA expression was quantitated by semi-quantitative RT-PCR.Results The expression of perilipin mRNA weren＇t seen in the 3T3-L1 preadipocytes.During differentiation,the perilipin mRNA expression of 3T3-L1 cells were increased along with time.In the differentiated 3T3-L1 adipocytes and differentiating 3T3-L1 cells,treatment of 3T3-L1 cells with 100 μmol·L-1 pioglitazone increased perilipin mRNA expression compared with untreated controls,while TNF-α decreased perilipin mRNA expression.Pioglitazone prevented the inhibitory effects of TNF-α on expression of perilipin mRNA in the 3T3-L1 adipocytes.Conclusions Along with the cell differentiation,the perilipin mRNA expression was increased in the 3T3-L1 cells.Pioglitazone enhanced perilipin mRNA expression in the 3T3-L1 cells,and TNF-α suppressed that.Pioglitazone prevented the inhibitory effects of TNF-α on expression of perilipin mRNA in the 3T3-L1 adipocytes.

关 键 词：PERILIPIN 吡格列酮 TNF-Α 3T3-L1脂肪细胞 分化 

分 类 号：R96[医药卫生—药理学]