丁苯酞对血管性痴呆大鼠认知功能、海马神经元结构及nNOS的影响  

Effects of butylphthalide on cognitive function,neurons of hippocampus histomorphology and the expression of nNOS in vascular dementia rats

作　　者：孙玉华[1] 卢宏[2] 

机构地区：[1]河南大学淮河医院神经内科,开封475000 [2]郑州大学第一附属医院神经内科,郑州450052

出　　处：《中国实用神经疾病杂志》2010年第15期17-19,共3页Chinese Journal of Practical Nervous Diseases

摘　　要：目的研究丁苯酞对血管性痴呆(VD)大鼠认知功能、海马神经元结构、及神经元型一氧化氮合酶(nNOS)的影响。方法采用结扎双侧颈总动脉方法制备慢性前脑缺血动物模型,100只老龄大鼠随机分5组,应用水迷宫、透射电镜及免疫组化方法对各组大鼠学习记忆、神经元结构、nNOS表达进行观察。结果与假手术组比较,大鼠学习记忆能力在造模后差异有显著性意义(P<0.05);大鼠海马区神经元在造模后变性水肿明显,与模型组比较,丁苯酞不同剂量治疗1月后,大鼠学习记忆能力明显改善(P<0.05),神经元变性水肿减轻,nNOS阳性神经元表达减少(P<0.05)。结论丁苯酞能显著改善VD大鼠学习记忆能力;减轻神经元变性水肿;抑制nNOS阳性神经元表达。Objective To observe the effects of butylphthalide on cognitive function and neurons of hippocampus histomor phology and on the expressions of Nitricoxidesyntbase （nNOS） positive cells in vascular dementia rats. Methods Model rats of chronic forebrain ischemia were subjected to permanent bilateral common carotid arteries ligation for 2 months. One hundred healthy 12- 14 month-old Wistar rats were randomly divided into five groups. Morris water maze was used to test the cognitive function of these rats. We observed neuron histomorphology in virtue of transmission electron microscope; nNOS positive cells were observed through immunohistochemistry technique. Results By comparison with sham operated group, the learning and memory abilities of model rats were obviously impaired （P〈0.05）. Neurons of hippocampus were dropsy and denaturalization significantly. After treatment of each dosage butylphthalide for one month, by comparison with model group the learning and memory abilities o[ VD rats were obviously improved （P〈0.05）. Dropsy and denaturalization of neurons were alieviative in the area of hippocampus, nNOS positive neurons decreased （P〈0.05）. Conclusion Butylphthalide may protect chronic cerebral ischemic injury; DBT can inhibit the activity of nNOS positive ceils to promote intelligence.

关 键 词：丁苯酞 血管性痴呆 神经元型一氧化氮合酶 大鼠 

分 类 号：R-332[医药卫生]