癫痫大鼠海马CA3区凋亡相关蛋白表达及意义  

Expression and significance of proteins associated with apoptosis in hippocampal CA3 Region in epilepsy rats

作　　者：刘晓梅[1] 尤红娟[1] 孙亚峰[1] 关秋华[1] 张光毅[1] 裴冬生[2] 

机构地区：[1]徐州医学院基础学院,江苏徐州221004 [2]徐州医学院肿瘤生物治疗重点实验室

出　　处：《山东医药》2010年第31期4-6,共3页Shandong Medical Journal

基　　金：国家自然科学基金资助项目(30800309);江苏省自然科学基金资助项目(BK2006536;BK2006035)

摘　　要：目的探讨癫痫大鼠神经元凋亡的分子机制。方法将SD大鼠分为观察组36只和对照组12只,观察组腹腔注射海人藻酸(KA)制作癫痫模型,对照组腹腔注射等量生理盐水。制模3、6、12h及1、3d采用免疫印迹法检测两组海马CA3区凋亡相关蛋白p-c-Jun、FasL、Bax、Bcl-2的表达;制模7d后采用TUNEL染色法检测CA3区神经元凋亡情况。结果制模6、12h观察组胞核内c-Jun的磷酸化和表达、胞质中FasL表达均明显高于对照组(P均<0.05);制模6h时Bax的表达急剧增加,而Bcl-2蛋白表达却明显降低,P均<0.05;制模7d后CA3区每1mm长度范围内TUNEL阳性细胞数为(177.0±24.7)个,对照组为(21.4±6.8)个,两组相比P<0.05。结论c-Jun介导的核通路和Bcl-2介导的非核通路共同参与了大鼠海马神经元的凋亡过程。Objective To investigate the molecular meachanism of neuron apoptosis in epilepsy rats. Methods SD rats were divided randomly into observation group （ 36 rats） and control group （ 12 rats） . Seizure model was prepared by intraperitoneal injection of kainite（ KA） in the observation group ,while the control group was treated with equal volume saline. Immunoblotting was performed to examine the expression of proteins associated with apoptosis such as p-c-Jun,FasL, Bax and Bcl-2 at 3 hs,6 hs,12 hs,1 d,3 ds after KA injection. TUNEL staining was used to examine the apoptosis of neuronal cells in hippocampal CA3 Region 7 ds after KA injection. Results Compared with the control group,the expression of p-c-Jun and FasL in the observation group increased significantly at 6hs and 12 hs after KA injection （ all P 0. 05） ; the expression of Bax in the observation group increased dramatically at 6h after KA injection,whereas the level of Bcl-2 decreased sharply（ all P 0. 05） ; 7 ds after KA injection,the number of TUNEL-positive cells/1mm of length in the observation group and control group were （ 177. 0 ± 24. 7） and （ 21. 4 ± 6. 8） pieces respectively,P 0. 05. Conclusion Activation of the nuclear and non-nuclear pathways mediated by c-Jun and Bcl-2 respectively participate jointly the neuron apoptosis in epilepsy rats .

关 键 词：癫痫 细胞凋亡 凋亡相关蛋白BCL-2 凋亡相关蛋白c-Jun FAS配体 BAX蛋白 

分 类 号：R742.1[医药卫生—神经病学与精神病学]