大豆对羟苯丙酮酸双加氧酶基因的克隆与表达分析  

Cloning and Expression Analysis of p-hydroxyphenylpyruvic Acid Dioxygenase Gene in Glycine max

作　　者：胡英考[1] 李雅轩[1] 蔡民华[1] 

机构地区：[1]首都师范大学生命科学学院,北京100048

出　　处：《大豆科学》2010年第4期569-574,共6页Soybean Science

基　　金：留学回国人员择优资助项目

摘　　要：采用电子克隆与实验克隆结合的方法获得了大豆对羟苯丙酮酸双加氧酶基因的cDNA序列,GenBank登录号为EF608178。利用多种生物信息学工具分析了该cDNA序列及其编码的蛋白质序列,分析了其启动子特征和电子表达谱。结果表明:该cDNA序列含有1个编码443个氨基酸的完整的开放读码框,3′端具有2个加尾信号和polyA尾巴。启动子区除含有通用核心元件外,还含有许多与光反应有关的作用元件;不同物种之间对羟苯丙酮酸双加氧酶的氨基酸序列同源性较高;该基因在光合作用和贮藏器官中的表达量高。A cDNA sequence of p-hydroxyphenylpyruvic acid dioxygenase gene was cloned by in silico cloning combined with experimental RT-PCR from Glycine max with a GenBank accessory number EF608178.Multiple bioinformation tools were used to analyze the cDNA and corresponding protein sequence,promoter characteristic and in silico expression pattern.Results showed that the cDNA has an intact open reading frame（ORF） encoding 443 amino acids.Two tailing signals and a polyA tail were found in 3′ region.Many light responsive elements were found in its promoter region including many common core promoter elements.Protein multiple-alignment and phylogenetic analysis suggestted that soybean HPPD has strong similarity in different plant species.in silico expression analysis results showed that its expression was high correlated with photosynthesis and store organs.

关 键 词：大豆 对羟苯丙酮酸双加氧酶 基因克隆 电子表达分析 

分 类 号：S565.1[农业科学—作物学]