无花果渣脂溶性物质的化学成分和体外抗肿瘤的活性研究  

Lipid-soluble Components from Fig Residue and Its Anti-tumor Activities in vitro

作　　者：王振斌[1] 马海乐[1] 马晓珂[1] 高宗颖[1] 常金科[1] 

机构地区：[1]江苏大学食品与生物工程学院

出　　处：《林产化学与工业》2010年第4期48-52,共5页Chemistry and Industry of Forest Products

基　　金：江苏省科技厅农业攻关项目(BE2002336)

摘　　要：以无花果残渣为原料,研究了无花果渣脂溶性物质抗肿瘤成分的提取和纯化方法,经过多次柱层析和波谱结构分析,得到13种单晶体。通过对13种单晶体对白血病细胞（U937）、肺癌细胞（95D）和胃癌细胞（AGS）的体外抗肿瘤试验,结果表明,晶体6的结构鉴定为β-谷甾醇,对肿瘤细胞株U937、95D和AGS抑瘤活性的半数抑制质量浓度（IC50）值小于10mg/L,具有抑瘤效果。提取无花果渣脂溶性抗肿瘤成分的最佳工艺为：提取溶剂为石油醚（沸程60～90℃）,果渣粒度750μm、提取温度85℃、提取时间130min、料液比1∶11（g∶mL）、提取2次,粗提物得率为23.1g/kg。Extraction and purification technology of lipid-soluble anti-tumor active components from fig residues were studied. Thirteen crystals were got after column chromatography, spectrometric study and structural analysis. The anti-tumor activity of these 13 crystals was investigated on blood tumor cell line（U937）, lung tumor cell line（95D）and stomach tumor cell line（AGS）in vitro. The Number 6 crystal was identified as β-sitosterol and validated as anti-tumor component for its fifty percent inhibition content （IC50）lower than 10mg/L. The optimum lipid-soluble anti-tumor components extraction condition are as follows, using petroleum ether（boiling point from 60 to 90℃） as solvent and the fig residues granularity 750μm, extracting temperature 85℃, extracting time 130min, the ratio of solid to solvent 1∶11（g∶mL）, extracting twice. As a result the anti-tumor substance extraction yield is 23.1g/kg. The anti-tumor component was identified as β-sitosterol after column chromatography, spectrometric study and structural analysis.

关 键 词：无花果 抗肿瘤 波谱分析 Β-谷甾醇 

分 类 号：TQ351[化学工程]