云南省禽流感、新城疫与3种免疫抑制性病原共感染的检测  

Detection of Mixed Infections of AIV,NDV and Three Kinds of Immunosuppressive Viruses in Yunnan Province

作　　者：宋建领[1] 石凤海 田建国[3] 叶丛华[3] 张文东[4] 赵焕云[4] 吕粤[4] 岳亮[4] 李华春[1] 邱薇[5] 冯子良[5] 张思东[4] 范泉水[5] 张应国[4] 张富强[5] 

机构地区：[1]云南省热带亚热带动物病毒病重点实验室,云南昆明650224 [2]临沧市动物疫病预防控制中心,云南临沧67000 [3]云南农业大学,云南昆明650223 [4]云南省动物疫病预防控制中心,云南昆明650051 [5]成都军区疾病预防控制中心,云南昆明650032

出　　处：《动物医学进展》2010年第8期24-29,共6页Progress In Veterinary Medicine

基　　金：云南省科技攻关项目(2006NG-24);云南省后备人才基金项目(2009CI061);云南省高层次科技人才培引工程(20080C002);农业部农业公益性行业科研专项(200803026)

摘　　要：为调查云南省禽流感病毒、新城疫病毒与免疫抑制性病毒混合感染的情况,对云南省2007年1月～2009年10月期间145份禽流感阳性及30份新城疫阳性样品进行禽网状内皮增生病病毒(REV)、J亚群禽白血病病毒(ALV-J)及鸡传染性贫血病毒(CIAV)的检测。禽流感阳性样品中ALV-J的感染阳性率为15.9%,REV的感染阳性率为22.8%,CIAV的感染阳性率为26.9%;新城疫阳性样品中ALV-J的感染阳性率为13.3%,REV的感染阳性率为23.3%,CIAV的感染阳性率为26.7%;共有30份样品发生多重感染,多重感染率为17.1%。对不同区域的4份禽白血病病毒阳性样品、10份禽网状内皮增生病病毒阳性样品、10份禽传染性贫血病毒阳性样品进行了序列比对和系统发育分析。结果表明,云南ALV-J毒株gp85基因与国内外毒株核苷酸及氨基酸同源性分别介于80.4%～96.7%、85.2%～94.5%;REV毒株env基因核苷酸及氨基酸之间的同源性为98.8%～99.8%、97.7%～99.5%;CIAV毒株vp2基因核苷酸及氨基酸之间的同源性为92.4%～100.0%、93.7%～100.0%。In order to investigate the mixed infections of AIV,NDV and immunosuppressive viruses in Yunnan province,145 positive samples for AIV and 30 positive samples for NDV were detected by RT-PCR for poultry Reticuloendothelial virus（REV）,J subgroup avian leukosis virus（ALV-J） and chicken infectious anemia virus（CIAV） from January 2007 to October 2009 in Yunnan Province.In positive for AIV samples,ALV-J infection rate was 15.9%,REV was 22.8% and CIAV was 26.9%;in positive for NDV samples,ALV-J infection rate was 13.3%,REV was 23.3% and CIAV was 26.7%;a total of 30 samples appeared multiple infections,the infection rate was 17.1%.4 positive ALV-J,10 poultry REV and 10 samples were used for sequence and phylogenetic analysis in different regions of Yunnan province.The results showed that：the nucleotide and amino acid homologies of ALV-J gp85 gene of Yunnan strain with domestic and overseas strains were ranged from 80.4%-96.7%,85.2%-94.5%;the nucleotide and amino acid homologies of env gene of REV strain were 98.8%-99.8%,97.7%-99.5%;the vp2 gene of CIAV strain were 92.4%-100.0%,93.7%-100.0%.

关 键 词：禽流感病毒 新城疫病毒 GP85基因 ENV基因 VP2基因 

分 类 号：S858.3[农业科学—临床兽医学]