乳汁中转基因蛋白表达的调节因子  

Regulating Factors of Transgenic Protein Expression in Breast Milk

作　　者：于宁[1] 刘耀川[2] 李明峰[2] 吴红涛[2] 李旭[2] 李树博[3] 屈楠[4] 张守纯[1] 

机构地区：[1]沈阳农业大学畜牧兽医学院,辽宁沈阳110161 [2]辽宁省重大动物疫病应急中心,辽宁沈阳110161 [3]辽宁省动物疫病预防控制中心,辽宁沈阳110164 [4]辽宁省动物卫生监测预警中心,辽宁沈阳110001

出　　处：《现代畜牧兽医》2010年第8期68-70,共3页Modern Journal of Animal Husbandry and Veterinary Medicine

摘　　要：激素调节所需的特异调节区域已经被证实,具有多绑定位点并可结合几个转录因子的复合应答元件已经得到确定。乳腺特异的基因表达的调节需要多个因子间相互作用的合作,由催乳素调节的信号转导通路引发了转录因子与调节元件的绑定和相互作用及乳蛋白基因的表达。β-酪蛋白和乳清酸蛋白mRNA的基因内5’序列和3’非翻译区分别对于基因的有效表达起到至关重要的作用。近年来,科学工作者们设计了许多种载体,可在乳腺中表达外源基因。这项技术可用于操纵转基因家畜的乳汁成分,利用乳腺作为生物反应器应用于生物制药学中。Specific regulatory regions required for hormonal regulation have been defined. Composite responso elements with multiple binding sites for several transcription factors have been identified. Mammary gland--specific gene expression mediated by cooperative interactions among several factors. Signal transduction pathways regulated by respectively, appear crucial for the efficient expression of these genes. Vectors to target the expression of heterologous genes to the mammary gland have been designed. This technology can be used to manipulate milk composition ir transgenic livestock and produce pharmaceutical protein by mammary gland bioreactor.

关 键 词：Β-酪蛋白 转录因子 复合应答元件 催乳素 糖皮质激素 

分 类 号：S813.3[农业科学—畜牧学]