黏蛋白分泌在双歧三联活菌减轻小鼠结肠炎复发中的作用  

Role of mucins secretion in the process that bifid triple viable capsule attenuates the recurrence of murine experimental colitis

作　　者：刘揆亮[1] 顾芳[1] 吕愈敏[1] 

机构地区：[1]北京大学第三医院消化科,100191

出　　处：《中华临床营养杂志》2010年第4期235-239,共5页Chinese Journal of Clinical Nutrition

摘　　要：目的观察黏蛋白分泌在双歧三联活菌减轻小鼠结肠炎复发中的作用。方法BALB／c小鼠分为双歧三联活菌干预组（BIFICO组）、阴性对照组[三硝基苯磺酸（TNBS）组]及正常对照组。BIFICO组及TNBS组小鼠于实验前5d起每Et分别予双歧三联活菌混悬液或磷酸盐缓冲液灌胃，实验当日以1．5％TNBS／50％乙醇溶液（125mg／kg）给予第1次灌肠，实验第5天于BIFICO组及TNBS组分别随机处死一部分小鼠，剩余小鼠以2．25％TNBS／45％乙醇溶液（180mg／kg）给予第2次灌肠。第7天实验结束时处死所有小鼠。实验过程中监测疾病活动指数（DAt）变化，行结肠炎症评分，并以免疫组化方法检测结肠黏膜内MUC2的表达。结果通过两次TNBS/乙醇溶液灌肠成功模拟了小鼠结肠炎复发。BIFICO组小鼠在第2次TNBS/乙醇溶液灌肠后，其DAI评分（1．39±0．68）及结肠炎症组织学评分（2．11±0．78）均明显低于TNBS组（分别为2．39±0．73及3．22±0．97）（P=0．003，P=0．03）；实验第5天第2次灌肠前处死的BIFICO组小鼠结肠黏膜内MUC2评分（8．67±0．87）高于TNBS组（7．86±1．10）（P=0．042）。结论双歧三联活菌可能通过促进黏膜内MUC2分泌从而增强黏膜屏障功能，进而减轻小鼠结肠炎复发的严重程度。Objective To investigate the role of mucins secretion in the process that bifid triple viable capsule attenuates the recurrence of murine experimental colitis. Methods Mice were randomly allocated into BIFICO group （garage with bifid triple viable capsule）, 2,4,6-trinitrobenzenesulfonic acid （TNBS） group （ga- rage with phosphate-buffered saline）, and normal control group. Intrarectal TNBS/ethanol challenge was given to mice on Day 0 followed by rechallenge on Day 5 （ 125 mg/kg and 180 mg/kg, respectively）. On Day 5 several mice in both groups were randomly sacrificed before the second TNBS challenge and the remaining mice were sacri- riced on Day 7. Disease activity index （DAI） was measured throughout the experiment. Inflammatory score was evaluated and MUC2 expression was determined using immunohistochemistry method. Results Recurrence of colitis was successfully simulated by challenging mice with two times of TNBS/ethanol solution. Compared with the TN- BS group, DAI （ 1.39 ± 0. 68 vs. 2. 39 ± 0. 73, P = 0. 003 ） and inflammatory score （2. 11 ± 0.78 vs. 3.22 ± 0. 97, P =0. 03） were significantly decreased in BIFICO group after the second TNBS challenge. BIFICO administration significantly increased MUC2 expression （8. 67 ± 0. 87 vs. 7. 86 ± 1.10, P = 0. 042 ） in BIFICO group compared with TNBS group before the second TNBS challenge. Conclusion Bifid triple viable capsule may enhance mueosal barriers by promoting mucins secretion, and thus attenuates the recurrence of murine colitis.

关 键 词：益生菌 黏蛋白 实验性结肠炎 复发 

分 类 号：R574.62[医药卫生—消化系统]