雷公藤红素对人肥大细胞白血病细胞系HMC-1作用的实验研究  

In vitro study on cellular and molecular mechanism of tripterine treating leukemic mast cells

作　　者：鲍一笑[1] 余润泉[1] 张登海[1] 李莉[1] 张玲珍[1] 徐玲玲[1] 孔宪涛[1] 

机构地区：[1]第二军医大学附属长征医院

出　　处：《中华血液学杂志》1999年第3期146-148,共3页Chinese Journal of Hematology

摘　　要：目的探讨雷公藤红素对人肥大细胞白血病的作用及机制。方法以人肥大细胞白血病细胞系ＨＭＣ１细胞为靶细胞，观察雷公藤红素对ＨＭＣ１细胞凋亡的诱导作用。结果①０．１２～１．００μｍｏｌ／Ｌ雷公藤红素可以引起ＨＭＣ１细胞凋亡，表现为细胞呈凋亡形态学改变，ＤＮＡ琼脂糖凝胶电泳出现梯形条带，流式细胞仪分析ＨＭＣ１细胞在Ｇ１期前出现凋亡峰；②凋亡率随药物浓度的增加及作用时间的延长而递增；③促进Ｇ１期细胞进入Ｓ期，使Ｓ期细胞比例升高，随后凋亡率升高，Ｓ期细胞下降，两者呈负相关（Ｐ＜０．０１）；④雷公藤红素可使ＨＭＣ１细胞ｂａｘ、ｃｍｙｃ表达增加，ｂｃｌ２表达下降。结论雷公藤红素可有效地诱导ＨＭＣ１细胞凋亡，凋亡主要发生在Ｓ期，凋亡的发生与其上调促进凋亡基因ｂａｘ、ｃｍｙｃ和下调抑凋亡基因ｂｃｌ２的表达有关。Objective To explore the effect of tripterine on leukemic mast cells. Methods The human leukemic mast cell line (HMC 1) was used as target cells for the tripterine’s effect. Results ①Apoptosis of HMC 1 cells could be efficiently induced by tripterine (0.125～1.0μmol/L), showing the apoptotic changes in morphology, DNA ladder on argarose gel electrophoresis and apoptotic peak before G 1 phase of cell cycle on flowcytometry. ② The magnitude of apotosis increased with the augmentation of tripterine concentration and duration of exposure; ③ With G 1 phase cells decreasing, S phase cells were increased, and then apoptotic cells increased with a diminution of S phase cells. They bored significant negative relation (P<0 01);④Tripterine could upregulate bax, c myc expression and downregulate bcl 2 expression at protein level. Conclusion Tripterine can efficiently induce HMC 1 cell apoptosis, occurring mainly in S phase, which is correlated with upregulating bax, c myc expression and downregulating bcl 2 expression.

关 键 词：白血病 肥大细胞 细胞凋亡 雷公藤红素 HMC-1 

分 类 号：R733.7[医药卫生—肿瘤] R979.1[医药卫生—临床医学]