兔脑缺血模型的影像学研究  

Imaging study of cerebral ischemic model on rabbits

作　　者：朱晓黎[1] 丁乙[1] 刘一之[1] 倪才方[1] 

机构地区：[1]苏州医学院附属第一医院影像中心

出　　处：《中华放射学杂志》1999年第3期168-170,共3页Chinese Journal of Radiology

摘　　要：目的通过研究兔颈动脉变异情况，探讨建立良好的适应临床影像学研究需要的脑缺血动物模型。方法４６只新西兰兔随机分为缺血组（４０只）和对照组（６只）。缺血组采用颈外动脉逆行置管，暂时夹闭颈总动脉，由导管内注入自体血凝块，建立脑缺血模型。对照组栓塞前手术过程与缺血组相同。缺血组在栓塞后不同时限行ＭＲＩＴ２ＷＩ检查及病理检查。结果４６只兔颈动脉变异共分为４型：Ⅰ型，单纯颈内动脉型（２６条，占５７％）；Ⅱ型，颈内动脉近端发出枕动脉分支型（１１条，占２４％）；Ⅲ型，颈内动脉远端发出枕动脉分支型（３条，占６％）；Ⅳ型，颈内动脉发出耳动脉分支型（６条，占１３％）。根据２４小时ＭＲＩＴ２ＷＩ所确定的梗死灶将３０只存活兔分为３型：局灶型（４４％），基底节和（或）小叶皮层型（３３％），以及半球型（２３％）。结论Ⅰ、Ⅱ、Ⅳ型适宜制作兔脑缺血模型，其中Ⅱ型需暂时夹闭枕动脉，Ⅲ型不适宜用于制作脑缺血模型。这种改进的脑缺血模型为临床影像学研究需要提供了实验基础。Objective To investigate the carotid artery variations of rabbit, we in order to establish a cerebral ischemic animal model which can adapt to the need of clinical study. Methods Forty six cases of rabbits are arranged into 2 groups: ischemic group (40 cases) and control group(6 cases). To set up the cerebral ischemic model, we retrogradely catheterized the external carotid artery, temporarily clipped the common carotid artery, then injected the autocoagnlated clot. The operation process of control group was the same as ischemic group before injecting the clot. MR T 2 weighted images and pathological histology examinations were done in ischemic group at different time span. Results Four types were classified according to variations: Type Ⅰ, simple internal carotid artery (57%); TypeⅡ, occipital artery arising from the proximal internal carotid artery (24%); TypeⅢ, occipital artery arising from the distal internal carotid artery(6%); Type Ⅳ, auricular artery arising from internal carotid artery(13%). Three types of ischemia were classified on the basis of the manifestations on MR T 2 weighted images: Focal type(44%); Basal ganlia and/or lobular cortex type(33%) and hemispheric type (23%). Conclusion Type Ⅰ, Ⅱ and type Ⅳ can be used for cerebral ischemic model whereas for type Ⅱ, temporary occlusion of the occipital artery was needed. Type Ⅲ can't be used as cerebral ischemic model. It is was concluded that the improved model provides a good experimental basis for the study of human ischemic stroke.

关 键 词：脑缺血 磁共振成像 实验研究 动物模型 

分 类 号：R743.31[医药卫生—神经病学与精神病学] R445.2[医药卫生—临床医学]