狼毒大戟根提取物抑菌活性及其提取方法初探  

Initial Studies on the Antifungal Activities and Extraction Method of Extracts from Euphorbia fischeriana Root

作　　者：孙瑞艳[1] 陈亚彤[1] 范海延[1] 于佳霖[1] 潘健[1] 

机构地区：[1]沈阳农业大学生物科学技术学院,辽宁沈阳110161

出　　处：《中国蔬菜》2010年第16期67-73,共7页China Vegetables

基　　金："十一五"国家科技支撑计划课题(2006BAD07B02);沈阳市大型科学仪器设备共享服务专项(2007GX-05)

摘　　要：以连续回流提取法从狼毒大戟根中提取得到抑菌活性物质,采用生长速率法进行离体抑菌试验确定其对多种植物病原真菌的抑菌活性。狼毒大戟根提取物对黄瓜枯萎病菌、菜豆炭疽病菌、甜瓜枯萎病菌等9种供试病原真菌菌丝生长均有一定的抑制作用。通过单因素试验和正交试验确定最优提取工艺为:以90%乙醇作为溶剂,料液比1︰20,在提取温度为65℃和提取溶剂pH为10.0条件下提取90min,提取次数为2次。In this paper,the bioactive substances obtained from Euphrbia fischeriana root were tested for antifungal activities against plant pathogens through growth rate law. The main factors influencing bioactive substances extraction and their best levels were confirmed by single factor analysis and the optimum condition was confirmed by orthogonal test. The research indicates that extracts of Euphrbia fischeriana root have inhibitory activities to many kinds of plant pathogen. In particular, inhibitory rate of Fusarium oxysporum f. sp. cucumerinum, Colletotrichum lindemuthianum and Fusarium oxysporum f. sp. melonis is better comparatively. The results showed that the optimal conditions were： 1∶20 of 90 % ethanol to sample weight, extraction temperature 65 ℃, pH value 10.0, extraction duration 90 min, and extraction for twice.

关 键 词：狼毒大戟 有机溶剂提取物 抑菌活性 黄瓜枯萎病菌 菜豆炭疽病菌 甜瓜枯萎病菌 

分 类 号：S482.292[农业科学—农药学]