琯溪蜜柚汁胞ACTIN的分离鉴定和cDNA克隆  

Isolation, Identification and cDNA Cloning of ACTIN of the Citrus grandis (L.) Osbeck Juice Sacs

作　　者：钟凤林[1] 郭志雄[1] 潘东明[1,2] 

机构地区：[1]福建农林大学园艺学院,福建福州350002 [2]福建农林大学园艺产品贮藏保鲜研究所,福建福州350002

出　　处：《热带作物学报》2010年第7期1124-1129,共6页Chinese Journal of Tropical Crops

基　　金：国家科技支撑计划项目(2007BAD07B01);福建省自然科学基金项目(2010J05047)资助

摘　　要：对琯溪蜜柚粒化与正常汁胞汁胞双向电泳分析,获得一个在粒化中上调表达的差异蛋白质点,经过质谱鉴定、生物信息学分析,为肌动蛋白(Actin),以琯溪蜜柚粒化汁胞cDNA为模板,根据Actin氨基酸序列设计引物,进行全长的扩增,得到Actin开放阅读框包含1 134个碱基,编码377个氨基酸。其核酸序列与黑杨派(Populus trichocarpa)、棉花(Gossypium hirsutum)、蓖麻(Ricinus communis)、圆叶锦葵(Malva pusilla)ACT的同源性在89%以上。The protein of Pomelo [Citrus grandis （L.） Osbeck] during different Juice Sac Granulation stages was separated and compared by IEF/SDS-PAGE technique. Action was one of the differential proteins identified by MALDI-TOF/TOF and bioinformatics analysis. Then Action cDNA sequence was cloned from Pomelo Juice Sac using RT-PCR technique. The open reading frame is 1 134 bp nucleotides, encoding a putative protein of 377 amino acid residues. The nucleotide sequence comparison with the Action gene of Populus trichocarpa, Gossypium hirsutum, Ricinus communis, Malva pusilla showed that identity was all higher than 89%.

关 键 词：琯溪蜜柚 ACTIN 双向电泳 串联质谱 克隆 

分 类 号：S666.3[农业科学—果树学]