龙血树DCDPK2基因的克隆及表达分析  

Cloning and Expression Analysis of Calcium-dependent Protein Kinase Gene in Dracaena Draco 2

作　　者：张浩[1,2] 蔡文伟[1] 张树珍[1] 杨学[1] 刘琳[1,2] 杨本鹏[1] 

机构地区：[1]中国热带农业科学院热带生物技术研究所农业部热带生物技术重点开放实验室,海南海口571101 [2]海南大学农学院,海南海口570228

出　　处：《热带作物学报》2010年第7期1130-1136,共7页Chinese Journal of Tropical Crops

基　　金：国家"863"重点项目(No.2007AA021504);中央级公益性科研院所基本科研业务费;海南省重点科技计划项目(No.080108)资助

摘　　要：通过RACE技术克隆龙血树CDPK基因的全长cDNA,并对基因序列进行相关分析与基因表达分析。结果表明:该基因全长为1 810 bp,编码区长度为1 587 bp,编码528个氨基酸,其氨基酸序列具有CDPK基因编码氨基酸的所有典型结构特征;经序列比对发现其核酸序列与玉米CDPK基因的同源性高达81%,氨基酸序列与其它植物中的CDPK氨基酸序列同源性很高。该基因命名为DCDPK2基因。对未经血竭诱导和诱导的龙血树组培苗进行半定量RT-PCR表达分析,发现在添加了诱导物的组培苗中DCDPK2基因的表达显著增强。The first time Calcium-dependent Protein Kinase genes was cloned by using RACE technique from Dracaena draco. The full length of the gene was 1 809 bp, the open reading frame encoding for 530 amino acids was 1593 bp long. Its encoded amino acid sequences had typical structural features from other CDPK genes. The nucleic acid sequence alignment revealed 81% identity with the ZmCDPK and its protein sequence showed high similarity with that from other plants. Semi-quantitative RT-PCR expression analysis with Dracaena tissue cultured plantlets found the expression of the gene DCDPK2 was markedly increased with the addition of the inducer.

关 键 词：龙血树 CDPK基因 克隆 RACE技术 表达分析 

分 类 号：S687.9[农业科学—观赏园艺] S567.19[农业科学—园艺学]