肠道病毒71型中国西安分离株全基因组核苷酸序列分析  

Complete Sequence Analysis of Enterovirus 71 XA87-2008 Isolate from Xi＇an,China

作　　者：卢晓玲[1] 郑海潮[2] 杜全丽[2] 黄银娟[2] 李一为[2] 杨扬[2] 李亚蓉[3] 马超锋[2] 

机构地区：[1]陕西省户县疾病预防控制中心,陕西户县710300 [2]西安市疾病预防控制中心,西安710054 [3]西安市儿童医院,西安710003

出　　处：《现代检验医学杂志》2010年第4期16-18,共3页Journal of Modern Laboratory Medicine

基　　金：陕西省卫生厅2008年卫生科研基金项目（08H22）.

摘　　要：目的 分析肠道病毒71型西安分离株（XA87-2008）全基因组核苷酸序列特征.方法 参考相关毒株序列,设计7对可重叠扩增全基因组的引物.提取一株2008年分离自西安市儿童医院手足口病病例标本的病毒基因组RNA,合成cDNA,进而扩增出7个目的片段,插入T载体克隆,对阳性克隆测序.利用MegAlign进行同源性分析,MEGA4软件进行进化分析.结果 XA87-2008毒株基因组全序7 414 bp,同源性分析与安徽阜阳2008年毒株高度同源,与其它基因亚型相差较大;进化分析显示其VP1区和全基因组序列均与C4基因亚型在同一进化支.结论 XA87-2008毒株为C4亚型,在进化关系上与近年来中国大陆流行株关系密切.Objective To analyse the genome characteristics of Enterovirus 71 XA87-2008 isolate. Methods Designed 7 overlapping primers to amplify the corresponding sequences of one EVTI isolate from hand-foot-mouth disease patient in Xi＇ an children hospital ,Shaanxi ,China,and inserted these sequences into T vectors ,then sequence the positive clones. Nucleotide homology and phylogenetic analysis were made by MegAlign and MEGA 4. Results Analysis showed that the isolate genome had 7 414bp and was most homologous to the strain circulating in Fuyang Anhui in 2008,and was C4 subgenotype. Conclusion Enterovirus 71 XA87-2008 isolate belong to C4 subgenotype and have close relation with the strains circulating in China's Mainland in these years.

关 键 词：肠道病毒71型 序列分析 同源性分析 系统进化 

分 类 号：R373.2[医药卫生—病原生物学] Q754[医药卫生—基础医学]