鹅Perilipin基因部分片段的克隆、不同品种及填饲对组织mRNA表达水平的影响  

Cloning of Goose Perilipin Gene,Tissues Expression and the Effect of Overfeeding on Its mRNA Level

作　　者：潘志雄[1] 王继文[1] 唐慧[1] 向梳瑕[1] 王静[1] 吕佳[1] 杨超[1] 韩春春[1] 

机构地区：[1]四川农业大学畜禽品种资源发掘与利用重点实验室,雅安625014

出　　处：《畜牧兽医学报》2010年第8期939-943,共5页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：国家科技支撑计划(2008BADB2B08)资助

摘　　要：本研究旨在克隆鹅围脂滴蛋白(Perilipin)基因,并探讨填饲对其在四川白鹅和朗德鹅肝脏中表达的影响,为从脂滴角度研究鹅肥肝分子机理提供依据。试验选取12只朗德鹅和12只四川白鹅为研究对象,通过RT-PCR方法扩增鹅Perilipin基因部分序列,采用实时定量技术研究了该基因在皮脂、腹脂、肝脏等10个组织中的表达情况,最后检测填饲后两品种鹅肝脏总脂质含量、甘油三酯(TG)水平、肝质量以及Perilipin基因表达变化。结果表明:获得的鹅Perilipin基因序列与原鸡的同源性最高,与哺乳动物的同源性也达到70%以上;从组织表达上看,该基因主要表达于腹脂和皮脂,而在其他组织几乎不表达;填饲引起鹅肝脏Perilipin mRNA表达丰度的极显著增加(P<0.01),且与肝质量、肝内TG和总脂质呈显著正相关;此外,填饲导致其在朗德鹅肝脏中的表达丰度显著高于四川白鹅(P<0.05)。结果提示:填饲会引起Perilipin mRNA在鹅肝脏中表达丰度的极显著增加(P<0.01),且其增加幅度存在着品种间差异(P<0.05)。This experiment was conducted to study the effect of overfeeding on Perilipin mRNA level in goose.In this study,the Landes goose and Sichuan White goose were used to clone the partial gene sequence of Perilipin,and the effect of overfeeding on the transcriptional level of Perilipin in liver was researched.The result indicated that the obtained Perilipin gene sequence was 277 bp,and had high similarity with other species.The mRNA expression of Perilipin gene was detected in different tissues of Sichuan White goose,and its expression level was higher in abdominal adipose and subcutaneous adipose tissues.Overfeeding markedly increased the mRNA expression of Perilipin gene in the liver of two breeds.In control group,the mRNA level of Perilipin in liver of Landes and Sichuan White goose was not different;compared with control group,in overfeeding group,the mRNA level of Perilipin in liver was markedly higher in Sichuan White goose and Landes goose,and there was significant difference at the mRNA level of Perilipin in liver between the two breeds.The mRNA abundance of Perilipin was positively correlated to the relative weight of liver,TG levels and total fatty acids in liver after overfeeding,and the correlation in Landes goose was stronger than that in Sichuan White goose.It was concluded that overfeeding induced the significant increase of Perilipin mRNA level in goose liver,and the effect of overfeeding was different between the two breeds.

关 键 词：鹅 填饲 围脂滴蛋白 基因克隆 MRNA表达 

分 类 号：S835[农业科学—畜牧学] Q756[农业科学—畜牧兽医]