宫颈脱落细胞中hTERC基因表达与宫颈病变及HPV感染的关系  

Relationship of human telomerase mRNA component gene expression in cervical exfoliated cells with cervical lesions and human papilloma virus infection

作　　者：何君梅[1] 尹格平[2] 卢金峰[2] 李红[1] 

机构地区：[1]云南省曲靖市第一人民医院妇产科,曲靖655000 [2]济南军区总医院,济南250031

出　　处：《第二军医大学学报》2010年第8期838-841,共4页Academic Journal of Second Military Medical University

基　　金：国家卫生部科研基金(WKJ2007-3-001)~~

摘　　要：目的探讨宫颈脱落细胞中人类染色体端粒酶mRNA基因(hTERC)扩增与宫颈病变程度及不同亚型人乳头瘤病毒(HPV)感染的关系。方法宫颈上皮内瘤样变(CIN)患者34例,其中CINⅠ8例,CINⅡ9例,CINⅢ(包括宫颈原位鳞癌)17例;浸润型宫颈鳞癌(ISCC)36例;慢性宫颈炎患者20例。对上述患者液基薄层细胞学检测(TCT)剩余样本应用荧光原位杂交技术(FISH)检测hTERC基因,快速导流杂交基因芯片技术检测HPV感染亚型,分析hTERC扩增与宫颈病变程度、HPV感染的关系。结果慢性宫颈炎、CINⅠ、CINⅡ、CINⅢ、ISCC中hTERC扩增率分别为0.00%(0/20)、50.00%(4/8)、77.78%(7/9)、82.35%(14/17)和97.22%(35/36),随着宫颈病变程度的增加,hTERC扩增率增高,组间两两比较差异均有统计学意义(P<0.05);慢性宫颈炎、CINⅠ、CINⅡ、CINⅢ、ISCC中HPV感染率分别为10.00%(2/20)、37.50%(3/8)、66.67%(6/9)、88.24%(15/17)、91.67%(33/36);HPV16型阳性、其他高危型阳性、阴性/低危型阳性组中hTERC扩增率分别为90.38%(47/52)、66.67%(4/6)、28.13%(9/32),组间比较差异具有统计学意义(P<0.01)。结论 hTERC扩增与宫颈病变的进展和HPV感染密切相关;HPV16型感染可能是导致高级别宫颈病变的主要原因,HPV58、33、52型在CIN及ISCC病变中也有一定优势作用。Objective To explore the relationship of human telomerase mRNA component gene（hTERC） expression with the degree of cervical lesions and the infection of different genotypes of human papilloma virus（HPV）.Methods The thinprep cytologic test（TCT） specimens from 34 pathologically confirmed cervical intraepithelial neoplasia（CIN,including 8 CINⅠ,9 CINⅡ,17 CIN Ⅲ）,36 invasive squamous cervical carcinoma（ISCC）,and 20 chronic cervicitis patients were included in the present study.HPV subtype infection was detected by channelization hybridization gene chip and hTERC expression was tested by fluorescence in situ hybridization（FISH）.The relationship between hTERC expression,the degree of cervical lesions and the infection with HPV was analyzed.Results The positive rates of hTERC in chronic cervicitis,CINⅠ,CINⅡ,CINⅢ,and ISCC specimens were 0.00%（0/20）,50.00%（4/8）,77.78%（7/9）,82.35%（14/17）,and 97.22%（35/36）,respectively.The positive rates of hTERC increased with the increase of cervical lesion degree,and there were significant differences between the three subgroups（P0.05）.The HPV infection rates were 10.00%（2/20）,37.50%（3/8）,66.67%（6/9）,88.24%（15/17）,and 91.67%（33/36）,respectively.The positive rates of hTERC in HPV16 type-positive,other high-risk type positive and negative/low-risk type positive groups were 90.38%（47/52）,66.67%（4/6）,and 28.13%（9/32）,respectively,with significant difference found between each two groups（P0.01）.Conclusion The progress of cervical lesions and HPV infection are closely related to the positive rates of hTERC.HPV16 infection is the main cause for the high-level cervical lesions,and HPV58,33,52 have some advantages in CIN or ISCC.

关 键 词：HTERC基因 人乳头瘤病毒 宫颈上皮内瘤样变 宫颈肿瘤 

分 类 号：R737.33[医药卫生—肿瘤]