湖北省36株单增李斯特菌脉冲场凝胶电泳分型  

Study on molecular typing by pulsed-field gel electrophoresis of food Listeria monocytogenes isolates from Hubei

作　　者：曾莹春[1] 郑华英[1] 吕均[2] 胡权[1] 熊燕[1] 马弋[3] 

机构地区：[1]武汉市疾病预防控制中心,武汉430015 [2]湖北省十堰市疾病预防控制中心,湖北十堰442000 [3]湖北省疾病预防控制中心,武汉430079

出　　处：《中国卫生检验杂志》2010年第8期1847-1849,共3页Chinese Journal of Health Laboratory Technology

摘　　要：目的:了解湖北省各类食品中分离到的单核细胞增生李斯特菌各菌株之间的遗传相关性,了解湖北省Lm基因型的分布情况和分子流行特点。方法:按照标准化的Lm-PGFE方法对所分离到的36株Lm菌株进行脉冲场凝胶电泳分型。结果:36株Lm菌株通过PGFE分成了12个型别,各型别之间不紧密相关。结论:湖北省食品中Lm的污染来源于不同的克隆株,菌型分散,未发现单个基因型菌株流行的迹象。Objective：This study is to investigate the genetic relativity and genotype distribution of Listeria monocytogenes strains separated from various foods in Hubei Province.Methods：According to international standardized Lm-PFGE protoco1,36 Lm strains isolated from foods were characterized by Pulsed-Field Gel Electrophoresis（PFGE）.Results：36 Lm strains isolated from Hubei province yielded 12 different genotypes by using PFGE,which had indirect association.Conclusion：The foodborne origin Lm strains are different clones which are distributed diffusedly in Hubei Province.No evidence indicates the prevalence of single genotype of Lm in Hubei.

关 键 词：单增李斯特菌 脉冲场凝胶电泳 分子分型 

分 类 号：R378.99[医药卫生—病原生物学]