毛细管柱气相色谱法测定饮料中环己基氨基磺酸钠  

Determination of sodium cyclamate in drinks by capillary gas chromatography

作　　者：王益萍[1] 陈海红[1] 沈仁富[1] 陈金斌[1] 陈宇鸿[1] 

机构地区：[1]浙江省温岭市疾病预防控制中心,浙江温岭317500

出　　处：《中国卫生检验杂志》2010年第8期1884-1885,共2页Chinese Journal of Health Laboratory Technology

摘　　要：目的:建立快速、简便测定饮料中环己基氨基磺酸钠的毛细管气相色谱检测方法。方法:样品在酸性条件下与亚硝酸钠反应,生成环己醇亚硝酸酯,通过HP-INNOWAX毛细管柱分离测定含量。结果:在0.10 mg/ml～4.00 mg/ml浓度范围内呈线性关系,回归方程为y=7E+06x-672551,相关系数为0.9997,相对标准偏差小于4.2%,加标回收率在90.0%～98.8%,最低检出浓度为5μg/ml。结论:本方法灵敏度高,检出限低,且分析时间短,可以用于大批量样品的检测分析。Objective：To develop a rapid and simple method for the determination of sodium cyclamate in drinks by capillary gas chromatography.Methods：The sodium cyclamate in sample was reactived with nitrous salt in acid medium,separated and determined by HP-INNOWAX capillary gas chromatography.Results：Beer′s law was obeyed in the range of 0.10 mg/ml～4.00 mg/ml,relational coefficient being r=0.9997.The relative standard deviation was below 4.2%.The recovery was between 90.0%～98.8%.Conclusion：The method was simple,rapid and sensitive with low detection limit,and it can be applied to the batch of sample analysis.

关 键 词：环己基氨基磺酸钠 毛细管气相色谱法 冰浴 衍生化 饮料 

分 类 号：O657.71[理学—分析化学]