绿色荧光蛋白基因标记的人乳腺癌细胞株MCF7-pAcGFP体外生长特性的研究  

Growth properties of human breast cancer cell line MCF7 - pAcGFP labelled with green fluorescent protein

作　　者：杨悦[1] 杨艳梅[1] 张春艳[1] 马玉彦[1] 蔡会龙[1] 任丽君[1] 

机构地区：[1]哈尔滨医科大学肿瘤防治研究所,哈尔滨150081

出　　处：《实用肿瘤学杂志》2010年第4期341-344,共4页Practical Oncology Journal

基　　金：黑龙江省科技厅资助项目（PC07S012）;省属科研院所自拟课题（2009-09）

摘　　要：目的研究增强型绿色荧光蛋白（pAcGFP）标记的人乳腺癌细胞株MCF7-pAcGFP的生长特性。方法应用Fugene HD Transfeetion Reagent转染MCF-7细胞，经G418筛选获得稳定表达pAcGFP的MCF-7细胞系。采用MTT方法检测MCF7-pAcGFP的增殖能力，采用流式细胞仪检测细胞周期，同时采用Western blot方法检测11种蛋白的表达情况。结果稳定表达pAcGFP的乳腺癌细胞株MCF7-pAcGFP的生长曲线和细胞周期分布与未转染前相似（P〉0．05），Cyclin D1、p27、Fas、FasL、Bcl-2、Bax、Akt、P—Akt（Ser473）、P—Akt（Thr308）、ERK以及P—ERK在两种细胞中表达情况无差别。结论与亲本细胞相比，MCF7-pAcGFP细胞株的生长特性并没有发生改变，为构建理想的动物模型来研究乳腺癌生长机制奠定了基础。Objective To investigate the growth properties of human breast cancer cell line MCF7 - pAcGFP labelled with green fluorescent protein. Methods Human breast cancer cell lines MCF - 7 were transfected with pAeGFP - C1 by Fugene HD Transtection Reagent. The cells which express the pAcGFP gene were selected by geneticin（G418）. The growth curve were detected by MTT,the cell cycle were detected by flow cytometry,and the expressions of 11 proteins in MCF7 - pAcGFP cells were detected by western blot. Results The growth curve and cell cycle of MCF7 - pAcGFP cells were similar to untransfected ones （ P 〉 0.05 ）. There were no significant diffrenee in the expression of Cyelin DI , p27, Fas, FasL, Bcl - 2, Bax, Akt, p - Akt （ Ser473 ） , p - Akt （ Thr308 ） , ERK and p - ERK between the MCF7 - pAcGFP cells and MCF - 7 cells. Conclusion Compa,＇ed with parents cells, there was no significant change in growth properties of MCF7 - pAcGFP cells. So MCF7 - pAcGFP cells may provide a basis for construction a ideal animal model to research molecular mechanisms of growth in human breast cancer.

关 键 词：绿色荧光蛋白 乳腺癌 转染 

分 类 号：R737.9[医药卫生—肿瘤]