近缘种扩增法对黑叶猴微卫星位点的筛选及特征分析(英文)  

Identification and characterization of microsatellite markers via crossspecies amplification from Francois'langur(Trachypithecus francoisi)

作　　者：孙涛[1,2] 王博石[2] 刘志瑾[2] 阙腾程[3] 黄乘明[1,2] 周岐海[1] 李明[2] 

机构地区：[1]广西师范大学珍稀濒危动植物与环境保护省部共建教育部重点实验室,桂林541004 [2]中国科学院动物研究所动物生态与保护生物学重点实验室,北京100101 [3]广西南宁市动物园,南宁530003

出　　处：《兽类学报》2010年第3期351-353,共3页Acta Theriologica Sinica

基　　金：funded by the key project of the Natural Science Foundation of China(NSFC,30970376,and30860050);Guangxi Nature Science Foundation(0991095);the Monitoring and Conservation of Langur Projects of the National Forestry Administration of China

摘　　要：微卫星位点近缘种筛选法使得在探讨各种灵长类种群遗传结构和生殖策略上更加便捷。我们利用138条人类微卫星引物在黑叶猴中进行筛选,得到了23个具有多态性位点。在28个检测个体中,每个位点的等位基因数为3到9个,期望杂合度为0.62,观测杂合度为0.50,其中有7个位点偏离Hardy-Weinberg平衡,9个位点存在无效等位基因现象。但是各位点之间均未检测到连锁不平衡现象。这些位点将在黑叶猴种群遗传结构的研究中发挥重要作用。Analysis of the population genetic structure and reproductive strategies of various primate species has been facilitated by cross-species amplification.We screened 138 human-derived markers to assess their utility in Francois＇langur（Trachypithecus francoisi）.Of the 138 loci,twenty-three produced reliable results and exhibited moderate levels of polymorphism.The number of alleles per locus ranged from three to nine among 28 individuals,and average observed and expected heterozygosities were 0.50 and 0.62,respectively.Seven loci showed significant deviation from Hardy-Weinberg equilibrium.There were null alleles at nine loci,but no linkage disequilibrium between loci was detected.These loci could be useful in the population genetic study of this species.

关 键 词：近缘种扩增 微卫星引物 黑叶猴 

分 类 号：Q75[生物学—分子生物学]