NF-κB圈套寡脱氧核苷酸联合紫杉醇对肺癌细胞增殖凋亡的影响  

NF-κB Decoy Oligonucleotide Technique (NF-κB decoy ODN) Combined with Paclitaxel on Apoptosis of Lung Cancer Cell Proliferation

作　　者：王宁[1] 邢丽华[1] 高炜[1] 高景[1] 焦婧[1] 赵世龙[1] 

机构地区：[1]郑州大学第一附属医院呼吸内科,河南郑州450052

出　　处：《医学与哲学（B）》2010年第8期41-42,48,共3页Medicine & Philosophy(B)

基　　金：河南省教育厅医学科技攻关计划项目;项目编号:2009A320041

摘　　要：探讨NF-κB圈套寡脱氧核苷酸(NF-κB decoy ODN)联合紫杉醇对肺癌细胞增殖凋亡的影响。培养人肺癌细胞A549:(1)脂质体瞬时转染细胞;(2)MTT试验观察细胞生长曲线;(3)流式细胞术检测细胞凋亡率。NF-κB decoyODN使细胞生长受到抑制,NF-κBdecoy ODN联合紫杉醇使细胞凋亡增加。NF-κB decoy ODN联合紫杉醇可增强对肺癌细胞增殖的抑制及凋亡的诱导。To investigate the NF-κB decoy oligodeoxy nucleotide combined with paclitaxel on apoptosis of lung cancer cell proliferation. Human lung cancer cell A549 ： liposomes transiently transfected cells. MTT test cell growth curve. Apoptosis rate by flow cytometry. NF-κB decoy ODN inhibited the cell growth. NF-κB decoy ODN plus paclitaxel to apoptosis. NF-κB decoy ODN plus paclitaxel may enhance lung cancer cell proliferation inhibition and apoptosis induction.

关 键 词：肺癌 NF—κB圈套寡脱氧核苷酸 紫杉醇 凋亡 增殖 

分 类 号：R734.2[医药卫生—肿瘤]