高效液相色谱手性流动相添加剂法拆分吡格列酮对映体  

Separation of Pioglitazone Enantiomers by HPLC with Chiral Mobile Phase Additives

作　　者：詹少卿[1] 杨燕[1] 张冬梅[1] 陈桂平[1] 

机构地区：[1]江苏南通市第一人民医院药剂科,南通市226001

出　　处：《中国药房》2010年第33期3156-3157,共2页China Pharmacy

摘　　要：目的:建立以高效液相色谱手性流动相添加剂法拆分吡格列酮对映体的方法。方法:通过筛选流动相中的有机修饰剂及用量、添加剂β-环糊精的浓度、缓冲盐的浓度、流动相pH值及柱温等建立高效液相色谱条件,并对进样后流出物进行定性鉴别。结果:建立的色谱条件中,色谱柱为Kromasil C18,流动相为水-乙腈(80∶20),其中含9mmo·lL-1β-环糊精、0.2%磷酸、10mmo·lL-1KH2PO4,pH值2.5,流速为0.6mL·min-1,检测波长为240nm,进样量为20μL,柱温为20℃。2种流出物经定性鉴别分别呈右旋和左旋。结论:成功分离出了吡格列酮的2种对映体。OBJECTIVE： To establish an HPLC with chiral mobile phase additives method to separate pioglitazone enantiomers. METHODS： The HPLC method was established by selecting organic modifier and its amount, the concentration of β-CD and buffer salt, pH value of mobile phase and column temperature. The effluent product was determined qualitatively. RESULTS： The HPLC condition was as follows： Kromasil C18 column was adopted with mobile phase consisted of water-acetonitrile （80 ∶ 20） containing 9 mmol·L-1 β-CD, 0.2% phosphoric acid, 0.01 mol·L-1 KH2PO4, pH=2.5. The flow rate was 0.6 mL·min-1. The detection wavelength was set at 240 nm and injection volume was 20 μL. The column temperature was 20 ℃. 2 kinds of effluent products were verified as left-wound spiral and right-wound spiral by qualitative identification. CONCLUSION： 2 kinds of enantiomers have been successfully separated from pioglitazone.

关 键 词：高效液相色谱法 吡格列酮 Β-环糊精 对映体分离 

分 类 号：R977.15[医药卫生—药品]