气相色谱-质谱联用检测保健品中的9种睾酮酯类  

Detection of Nine Testosterone Agents in Nutriment Products by GC/MS

作　　者：王占良[1] 张建丽[1] 张亦农[1] 

机构地区：[1]国家体育总局中国反兴奋剂中心,北京100029

出　　处：《现代科学仪器》2010年第4期90-93,共4页Modern Scientific Instruments

摘　　要：采用气相色谱-质谱联用法同时检测保健品中的9种睾酮酯类(睾酮Testosterone(T),醋酸睾酮T acetate,丙酸睾酮T propionate,异己酸睾酮T isocaproate,庚酸睾酮T enantate,环戊丙酸睾酮T cypionate,葵酸睾酮T decanoate,苯丙酸睾酮T phenpropionate,十一酸睾酮T undecylate)。采用叔丁基甲醚提取,针对保健品剂型的不同分别采取C-18反相固相萃取柱和硅胶正相固相萃取柱分离纯化,甲基睾酮作为内标,采用HP-1MS柱(17m×0.2 mm i.d.×0.11mm)色谱分离,程序升温,质谱检测。该方法的检测限和回收率均满足保健品的日常检测要求,前处理简单、快速、可靠。Nine testosterone esters （Testosterone, Testosterone acetate, Testosterone propionate, Testosterone isocaproate, Testosterone ceantate, Testosterone cypionate, Testosterone decanoate, Testosterone phenproionate and Testosterone undecylate） were simultaneously detected by gas chromatographymass spectrometry coupling with electronic ionization. The sample was extracted and purified by solid prepared extraction column and completely separated by HP-1MS colunm （17m ×0.2 mm i.d. ×0.11mm thickness） in linear temperature program, methyltestosterone as internal standard. The method is simple, rapid and reliable. It is suitable for routine analysis in nutriment products.

关 键 词：保健品 气相色谱-质谱 睾酮 

分 类 号：TS218[轻工技术与工程—粮食、油脂及植物蛋白工程]