江苏省糯玉米籽粒淀粉黏度性状与SSR标记的关联分析  

Association analysis of starch pasting characteristic traits with SSR markers in waxy corn in Jiangsu province

作　　者：张丹[1] 刘丽君[1] 薛林[2] 李建 徐辰武[1] 

机构地区：[1]扬州大学江苏省作物遗传生理重点实验室,江苏扬州225009 [2]江苏沿江地区农业科学研究所,江苏如皋226541 [3]如皋市农业科学研究所,江苏如皋226500

出　　处：《扬州大学学报（农业与生命科学版）》2010年第2期36-42,F0003,共8页Journal of Yangzhou University：Agricultural and Life Science Edition

基　　金：国家重点基础研究发展计划项目(973-2006CB101700);江苏省高校自然科学研究重大项目(09KJA210002);江苏省农业科技自主创新资金项目[CX(09)637];江苏省高新技术研究计划项目(BG2005309)

摘　　要：以53份糯玉米自交系和30份单交种为试验材料,测定其籽粒淀粉黏度,并使用91对SSR标记对这些材料进行基因组扫描。在检测群体结构的基础上使用TASSEL软件的GLM方法进行关联分析。结果表明:①供试材料在7个淀粉黏度性状上均呈极显著差异。②自交系中共扩增出433个差异片段,平均每对引物扩增出5个;引物PIC值在0～0.841之间,平均为0.573。单交种中扩增出403个差异片段,平均每对引物扩增出4个,引物PIC值在0～0.841之间,平均为0.562。③表型数据聚类结果与分子标记聚类结果存在一定的相似性。④经线性整合的SSR位点组合间存在一定的连锁不平衡,不平衡程度D′大于0.5的组合数占总组合数的10.31%。⑤遗传结构分析将供试群体分为9个亚群体,共发现26个SSR标记与籽粒淀粉黏度性状相关联。⑥有11个SSR标记同时与2个或2个以上性状相关联,可能与这些性状高度正相关或一因多效有关。⑦关联标记对表型变异的总解释率较高,部分性状的解释率总和超过100%。53 waxy corn inbreds and 30 waxy corn hybrids were selected as materials.Based on the data of starch pasting characteristic traits,analysis of phenotypic variation was carried out.91 pairs of SSR polymorphism markers were used to scan the genome of waxy corn.With the genotype and phenotype data genetic diversity analysis were also carried out.To perform association analysis,53 waxy corn inbreds and 30 waxy corn hybrids were combined as one population and analyzed for linkage disequili-brium（LD） of pairwise loci and population structure,then for association between SSR loci and starch pasting characteristic traits under TASSEL GLM（general linear model） program.The results were as follow： ① Differences among the tested materials were very significant in starch pasting characteristic traits.② 433 specific DNA bands were detected in waxy corn inbreds.1～10 polymorphic fragments were amplified stably by each primer with an average of 5 fragments.The value of polymorphism information content（PIC） for each SSR locus varied from 0 to 0.841 with an average of 0.573.While in hybrids,403 specific DNA bands were detected.1～10 polymorphic fragments were amplified by each primer with an average of 4 fragments.The value of PIC for each SSR locus varied from 0 to 0.841 with average of 0.562.③ The cluster results of starch pasting characteristic traits and SSR markers had a certain degree of similarity.④ LD was detected among syntenic SSR markers and the loci pairs with D′〉0.5 was 10.31% of the total.⑤ Genetic structure analysis showed that the combined population was composed of 9 subpopulations.The 26 SSR loci with a total of 91 loci were found to be associated with 7 starch pasting characteristic traits.⑥ There were 11 loci associated simultaneously with two or more traits,which might be the genetic reason of positive correlation among traits as well as the pleiotropic effects of gene（s）.⑦ The total interpretation rate of phenotypic variability of markers associated with starch pasting ch

关 键 词：糯玉米 淀粉黏度性状 遗传多样性 SSR标记 关联分析 

分 类 号：S513[农业科学—作物学]