不同培养条件下滑菇菌株酯酶同工酶的研究  

Study on the esterase isoenzyme in different culture condition of Pholiota nameko

作　　者：宗立立[1] 宋慧[1] 付岩松[2] 廖鸳鸯[1] 李壮[1] 

机构地区：[1]吉林农业大学生命科学学院,吉林长春130118 [2]沈阳农业大学生物科学技术学院,辽宁沈阳110866

出　　处：《扬州大学学报（农业与生命科学版）》2010年第2期78-81,共4页Journal of Yangzhou University：Agricultural and Life Science Edition

基　　金：国家"十一五"科技支撑计划项目(2007BAI38B06)

摘　　要：采用聚丙烯酰胺凝胶电泳(PAGE)技术,分析固体培养基和液体培养基不同时间对滑菇菌株酯酶同工酶酶带的影响以及固、液2种培养方式下滑菇10个菌株间酯酶同工酶酶谱多态性,并采用聚类分析法确定菌株间亲缘关系。结果表明:不同固体培养基种类和液体不同培养时间下,滑菇菌株酯酶同工酶酶谱呈现不同的多态性,PDA固体培养基、液体培养13 d的同工酶酶谱可有效鉴定各菌株,其聚类分析结果基本一致。Polyacrylamide gel electrophoresis（PAGE） was used to analyse the effect of two kinds of solid medium and different culture time in liquid medium on the esterase zymogram polymorphisms of Pholiota nameko strains,and the changes of esterase zymogram polymorphisms of 10 Pholiota nameko strains cultured in solid and liquid medium.Also,the genetic relationship among strains was identified by cluster analysis.The results showed that there existed different zymogram polymorphisms in two kinds of solid medium and different culture time in liquid medium.The esterase isozyme zymogram of strains in PDA solid medium and 13 d cultured in liqid medium can be used for identification of Pholiota nameko strains.Results of cluster analysis of the esterase isozyme zymogram in above two kinds of conditions were similar to each other.

关 键 词：滑菇 酯酶同工酶 聚丙烯酰胺凝胶电泳 酶谱 聚类分析 

分 类 号：S646.16[农业科学—蔬菜学]