大鼠脑缺血再灌注后核不均一性核糖核蛋白(hnRNP)A2/B1在脑皮质的表达变化  

Expression of heterogeneous nuclear ribose nucleoprotein A2/B1 in cerebral cortex of rats after cerebral ischemia/reperfusion

作　　者：刘一沉[1,2] 李保全[2] 张效东[2] 尹军[2] 白侠[2] 井丽枝[2] 张成岗[3] 王航雁[1] 

机构地区：[1]解放军总医院小儿内科,北京100853 [2]解放军第159医院小儿内科,河南驻马店463000 [3]军事医学科学院放射与辐射研究所,北京100850

出　　处：《军医进修学院学报》2010年第9期904-906,909,共4页Academic Journal of Pla Postgraduate Medical School

基　　金：国家自然科学基金(30973107);神经生物学国家重点实验室基金(No09-02)~~

摘　　要：目的探讨大鼠脑缺血再灌注损伤后核不均一性核糖核蛋白(Heterogeneous Nuclear Ribonulcleoprotein,hnRNP)A2/B1的表达变化与再灌注损伤时间关系。方法建立大鼠脑缺血再灌注损伤动物模型,检测缺血再灌注损伤后3、6、12、24、48h、72h在缺血侧大脑皮质四个区域hnRNPA2/B1含量变化,并与假手术对照组进行比较。结果与假手术组比较,hnRNPA2/B1表达量在脑缺血再灌注3h、6h、12h、24h明显下降(P<0.01)。48h后,hnRNPA2/B1蛋白表达量明显上升(P<0.01)。结论hnRNPA2/B1可能在基因转录水平参与保护调控缺血再灌注脑损伤。Objective To study the correlation between the expression of heterogeneous nuclear ribose nucleoprotein（hnRNP） A2/B1 in cerebral cortex of rats and the time of cerebral ischemia/reperfusion injury.Methods Ischemia/reperfusion injury was observed by detecting the hnRNP A2/B1 levels in 4 areas of cerebral cortex 3,6,12,24,48 and 72h after a rat model of cerebral ischemia/reperfusion injury was established,and compared with that in sham operation group.Results The expression level of hnRNP A2/B1 was significantly lower 3,6,12,and 24h after cerebral ischemia-reperfusion injury,and significantly higher 48h after cerebral ischemia-reperfusion injury in experimental groups than in sham operation group（P0.01）.Conclusion hnRNP A2/B1 may be involved in regulation of cerebral ischemia/reperfusion injury at gene transcription level.

关 键 词：核不均一性核糖核蛋白 再灌注损伤 脑损伤 大鼠 

分 类 号：R36-33[医药卫生—病理学]