HPLC法测定鸭跖草中异荭草素的含量研究  

Study on Determination of Iso-orientin in Commelina communis L. by HPLC-UV

作　　者：刘萍萍[1] 王冰[1] 

机构地区：[1]辽宁中医药大学药学院,辽宁大连116600

出　　处：《现代中药研究与实践》2010年第5期74-75,共2页Research and Practice on Chinese Medicines

摘　　要：目的研究中药鸭跖草中黄酮类成分异荭草素的含量测定方法,并比较不同产地生态环境鸭跖草中异荭草素的含量。方法采用AgilentTC-C18(4.6 mm×250 mm,5μm)色谱柱,以乙腈-0.1%磷酸溶液(15:85)为流动相,流速为1 mL·min-1,紫外检测器检测。结果异荭草素的线性范围为0.08~1.00μg,平均回收率为101.2%,不同产地不同生态环境样品的异荭草素含量相差有4倍之多。结论该方法便捷、有效,适用于检测鸭跖草中异荭草素含量,为其质量标准的制定提供参考。Objective To establish a method for determination the contents of iso-orientin in Commelina communis L.and to compare the determinations on different collection period.Methods The Agilent TC-C18（4.6 mm×250 mm,5 μm） was used,the acetonitrile-0.1%phosphoric acid solution（15：85）as mobile phase,the flow rate was 1 mL？min-1 with UV detected.Results The linear ranges of were at 0.08 μg^1.00 μg,the average recoveries were 101.2%,differences of ecological environmental samples from different areas are four times.Conclusion The method is convenient and effective.It can provide references for quality standards.

关 键 词：鸭跖草 HPLC-UV 异荭草素 含量测定 

分 类 号：S336[农业科学—作物遗传育种]