秦川牛CEBPA基因遗传变异对体尺和胴体性状的影响  

Effect of Genetic Variation of Qinchuan Cattle CEBPA Gene on Body Measuremente and Carcass Traits

作　　者：贺花[1] 刘小林[1] 顾玉兰[1] 刘宇[1] 王国庆[1] 胡如久[1] 

机构地区：[1]西北农林科技大学动物科技学院,陕西省农业分子生物学重点实验室,陕西杨凌712100

出　　处：《中国畜牧杂志》2010年第17期1-5,共5页Chinese Journal of Animal Science

基　　金：国家高技术研究发展计划“863”(2008AA101010);陕西省13115科技创新工程重大专项(2008ZDKG-11)

摘　　要：试验以CCAAT增强子结合蛋白A(CEBPA)为候选基因,采用直接测序的方法寻找秦川牛CEBPA基因SNP,筛查到一个新的多态位点T963G,该突变位于基因的编码区。运用PCR-RFLP方法验证并分析该位点在215头秦川牛群体中的多态性。结果表明:在秦川牛群体中分布A、B 2个等位基因,处于中度多态。经过χ2适合性检验,秦川牛群体在该位点达到Hardy-Weinberg平衡状态(P>0.05)。将该T963G位点的基因型与体尺、胴体性状进行关联分析,表明该位点与胴体重显著相关(P<0.05),BB为有利基因型,B等位基因为有利等位基因,提示该位点有可能作为秦川牛胴体性状标记辅助选择的标记之一。CCAAT enhancer binding protein A（CEBPA） gene was studied as a candidate gene for the body measurement and carcass traits of bovine.A new SNP T963G was detected by sequencing at CDS region.Different genotypes were determined in Qinchuan cattle by restriction fragment length polymorphism（RFLP）.The value of polymorphism information content indicated that it was a moderate polymorphism in Qinchuan cattle.The χ^2 test indicated that the polymorphic locus in Qinchuan cattle did fit Hardy-Weinberg equilibrium（P 〉0.05）.T963G polymorphism significantly associated with carcass weight（P〈 0.05）g,enotype BB was a predominant genotype and B was a predominant allele.It is suggested that T963G locus may be one of the marker-assisted selection used as carcass traits in Qinchuan cattle.

关 键 词：CEBPA基因 PCR-RFLP 体尺性状 胴体性状 秦川牛 

分 类 号：S823.2[农业科学—畜牧学]