趋化因子CCL20及其受体CCR6在急性坏死性胰腺炎中的表达及意义  

Expression and role of CC chemokine ligand 20 and CC chemokine receptor 6 in the pancreas of rats with acute necrotizing pancreatitis

作　　者：周国雄[1] 周德霞[1] 丁晓凌[1] 张海峰[1] 张弘[1] 成建萍[1] 强晖[1] 魏群[1] 华国平[1] 

机构地区：[1]南通大学附属医院消化科,江苏南通226001

出　　处：《中华胰腺病杂志》2010年第4期263-266,共4页Chinese Journal of Pancreatology

基　　金：南通市社会发展基金资助项目（$5054）

摘　　要：目的探讨CC趋化因子配体20（CCL20）和CC趋化因子受体6（CCR6）在实验性急性坏死性胰腺炎（ANP）发病机制中的作用。方法48只SD大鼠按完全随机法分为ANP组和对照组。采用胆胰管逆行注射4％牛磺胆酸钠建立大鼠ANP模型，以注射等容积生理盐水作为对照组。术后1、3、6、12h分批处死大鼠，取血检测血清淀粉酶，胰腺组织常规病理检查并评分，免疫组化和RT—PCR检测胰腺组织中CCL20、CCR6mRNA及蛋白表达。结果ANP大鼠血清淀粉酶和胰腺组织病理评分明显高于对照组。ANP组术后胰腺组织CCL20 mRNA及蛋白表达呈时间依赖性增强（P〈0．05）；术后6h胰腺组织中CCR6mRNA明显高于对照组（0．88±0．05对0．23±0．09，P〈0．01），术后12h CCR6 mRNA表达较6h时降低，但仍高于对照组（0．37±0．10对0．15±0．07，P〈0．05），CCR6蛋白变化与其mRNA变化一致。结论CCL20和CCR6参与ANP的发生和发展。Objective To investigate the role of CC chemokine ligand 20 （CCL20） and CC chemokine receptor 6 （ CCR6 ） in the pathogenesis of acute neerotizing pancreatitis （ANP）. Methods 48 SD rats were randomly divided into two groups： control group and ANP group. The ANP model was induced by retrograde infusion of 4 % sodium taurocholate into the biliary and pancreatic duct in SD rats. The same amount of saline was injected in the control group. The rats were sacrificed at 1, 3, 6, 12 h, the serum amylase levels and the pathological score of the pancreas were measured. The expressions of CCL20 and CCR6 mRNA and protein in pancreas were detected by immunohistochemistl3, and semi-quantitative RT-PCR, respectively. Results The levels of serum amylase and the histological score of ANP group were significantly higher than those of control group （P 〈 0.01 ）. The expression of pancreatic CCL20 mRNA and protein was increased in a time-dependant manner （P 〈 0.05）. The expression of pancreatic CCR6 mRNA at 6h was significantly higher than that of control group （0.88±0.05 vs 0. 23±0.09, P 〈0.01 ）. The expression of pancreatic CCR6 mRNA at 12h was decreased when compared with that of 6h group, but it was still higher than that of control group （0.37 ±0.10 vs 0. 15 ± 0.07, P 〈 0.05 ） , the change of CCR6 protein was consistent with that of CCR6 mRNA. Conclusions CCL20 and CCR6 may play an important role in the pathogenesis of ANP.

关 键 词：胰腺炎 急性坏死性 趋化因子 CC 趋化因子受体 

分 类 号：R576[医药卫生—消化系统]